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  Regulatory changes of membrane transport and ouabain binding during progesterone-induced maturation ofXenopus oocytes

Richter, H.-P., Jung, D., & Passow, H. (1984). Regulatory changes of membrane transport and ouabain binding during progesterone-induced maturation ofXenopus oocytes. Journal of Membrane Biology, 79, 203-210. doi:10.1007/BF01871059.

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 Creators:
Richter, Hans-Peter1, Author              
Jung, Dieter1, Author              
Passow, Hermann1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              

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Free keywords: Na-K ATPase; ouabain; Xenopus; oocyte; maturation
 Abstract: uring progesterone-stimulated maturation of defolliculated full-grownXenopus oocytes, the activities of the transport systems forl-alanine, thymidine, chloride, phosphate, and alkali ions decrease. Differences of the extent and time course of these changes suggest that they are controlled by at least partially independent mechanisms. A closer investigation of the Na-K ATPase has shown that in unstimulated oocytes, ouabain produces maximal inhibition when 8–12×109 molecules are bound per cell. This number is bound during the first phase of a diphasic uptake process. Since this phase can be suppressed by increasing the concentration of external K+ to 45 mmol/liter or more, it is concluded that it refers to binding to the Na−K pump in the plasma membrane. Ouabain bound prior to progesterone-induced germinal vesicle breakdown (GVBD) remains bound after the breakdown, although the Na−K pump loses the capacity to bind ouabain after GVBD in oocytes that had not been exposed to ouabain preceding GVBD. In the presence of Mg++ membranes isolated before regulatory inhibition of pumping and ouabain binding show a Na+-dependent incorporation of 32P from γ-[32P]-ATP that can be reversed by the addition of K+. The phosphorylation site migrates on LiDS-polyacrylamide gel electropherograms at about 98,000 daltons and can be identified as a Commassie blue-stainable band.

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Language(s): eng - English
 Dates: 1984-01-241984-10-01
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/BF01871059
 Degree: -

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Title: Journal of Membrane Biology
  Other : J. Membr. Biol.
Source Genre: Journal
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Publ. Info: New York : Springer-Verlag New York
Pages: - Volume / Issue: 79 Sequence Number: - Start / End Page: 203 - 210 Identifier: ISSN: 0022-2631
CoNE: https://pure.mpg.de/cone/journals/resource/954925415943