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  Structural characterization of fondaparinux interaction with per-6-amino-beta-cyclodextrin: An NMR and MS study

Várnai, B., Grabarics, M., Szakács, Z., Pagel, K., Malanga, M., Sohajda, T., et al. (2021). Structural characterization of fondaparinux interaction with per-6-amino-beta-cyclodextrin: An NMR and MS study. Journal of Pharmaceutical and Biomedical Analysis, 197: 113947. doi:10.1016/j.jpba.2021.113947.

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2021
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 Urheber:
Várnai, Bianka1, Autor
Grabarics, Márkó2, 3, Autor           
Szakács, Zoltán4, Autor
Pagel, Kevin2, 3, Autor           
Malanga, Milo5, Autor
Sohajda, Tamás5, Autor
Béni, Szabolcs1, Autor
Affiliations:
1Semmelweis University, Department of Pharmacognosy, Üllői út. 26, H-1085, Budapest, Hungary, ou_persistent22              
2Freie Universität Berlin, Institute of Chemistry and Biochemistry, Arnimallee 22, 14195, Berlin, Germany, ou_persistent22              
3Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              
4Gedeon Richter Plc., Spectroscopic Research Department, H-1475, Budapest, P.O.B. 27, Hungary, ou_persistent22              
5CycloLab, Cyclodextrin R&D Ltd, Budapest, H-1097, Illatos út 7, Hungary, ou_persistent22              

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Schlagwörter: Heparin NMR Electrostatic interaction Antidote Degradation
 Zusammenfassung: The highly anionic synthetic pentasaccharide fondaparinux (FDPX) – representing the antithrombin binding sequence of heparin – is in clinical use as a potent anticoagulant. Contrary to the unfractionated heparin, FDPX lacks potent antidote completely reversing its anticoagulant activity, therefore it is of great importance to identify new structures exhibiting strong intermolecular interactions towards FDPX. The polycationic heptakis(6-amino-6-deoxy)-beta-cyclodextrin (NH2-β-CD) can serve as an excellent model compound to mimic these interactions between the oppositely charged oligosaccharides. Herein, extensive NMR spectroscopic and nano-electrospray ionization mass spectrometric (nESI-MS) studies were conducted to understand the molecular-level interactions in the FDPX - NH2-β-CD systems. NMR experiments were performed at pD 7.4 and 2.0. Job’s method of continuous variation and 1H NMR titration experiments suggested the formation of FDPX∙NH2-β-CD complex at pD 7.4, while the presence of multiple complexes was assumed at pD 2.0. Stability constants were determined by separate 1H NMR titrations, yielding log β11=3.65 ± 0.02 at pD 7.4, while log β11 ≥ 4.9 value suggested a high-affinity system at pD 2.0. 2D NOESY NMR studies indicated spatial proximities between the anomeric resonance α-l-iduronic acid residue and the cyclodextrin’s methylene unit in the proximity of the cationic amino function. Acidic degradation of FDPX was investigated by NMR and MS for the first time in detail confirming that desulfation occurs involving one to two sulfate moieties. The desulfation of FDPX was inhibited by the cationic cyclodextrin in the case of equimolar ratio at pD 2.0. This is the first report on the stabilizing effect of cyclodextrin complexation on heparin degradation.

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Sprache(n): eng - English
 Datum: 2020-10-162021-01-302021-02-032021-04-15
 Publikationsstatus: Erschienen
 Seiten: 10
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/j.jpba.2021.113947
 Art des Abschluß: -

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Titel: Journal of Pharmaceutical and Biomedical Analysis
  Andere : Journal of Pharmaceutical and Biomedical Analysis
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Oxford : Elsevier
Seiten: 10 Band / Heft: 197 Artikelnummer: 113947 Start- / Endseite: - Identifikator: ISSN: 0731-7085
CoNE: https://pure.mpg.de/cone/journals/resource/954925534045