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  Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense

Zwiener, T., Mickoleit, F., Dziuba, M., Rückert, C., Busche, T., Kalinowski, J., et al. (2021). Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense. BMC Microbiology, 21(1): 65. doi:10.1186/s12866-021-02124-2.

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 Creators:
Zwiener, Theresa, Author
Mickoleit, Frank, Author
Dziuba, Marina, Author
Rückert, Christian, Author
Busche, Tobias, Author
Kalinowski, Jörn, Author
Faivre, Damien1, Author              
Uebe, René, Author
Schüler, Dirk, Author
Affiliations:
1Damien Faivre, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863290              

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Free keywords: Magnetospirillum gryphiswaldense; Magnetosomes; Genome reduction
 Abstract: Magnetosome formation in the alphaproteobacterium Magnetospirillum gryphiswaldense is controlled by more than 30 known mam and mms genes clustered within a large genomic region, the ‘magnetosome island’ (MAI), which also harbors numerous mobile genetic elements, repeats, and genetic junk. Because of the inherent genetic instability of the MAI caused by neighboring gene content, the elimination of these regions and their substitution by a compact, minimal magnetosome expression cassette would be important for future analysis and engineering. In addition, the role of the MAI boundaries and adjacent regions are still unclear, and recent studies indicated that further auxiliary determinants for magnetosome biosynthesis are encoded outside the MAI. However, techniques for large-scale genome editing of magnetic bacteria are still limited, and the full complement of genes controlling magnetosome formation has remained uncertain.

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Language(s): eng - English
 Dates: 2021-02-252021
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1186/s12866-021-02124-2
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Title: BMC Microbiology
Source Genre: Journal
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Publ. Info: London : BioMed Central
Pages: - Volume / Issue: 21 (1) Sequence Number: 65 Start / End Page: - Identifier: ISSN: 1471-2180