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  AtFUT4 and AtFUT6 are arabinofuranose-specific fucosyltransferases

Soto, M. J., Prabhakar, P. K., Wang, H.-T., Backe, J., Chapla, D., Bartetzko, M. P., et al. (2021). AtFUT4 and AtFUT6 are arabinofuranose-specific fucosyltransferases. Frontiers in Plant Science, 12: 589518. doi:10.3389/fpls.2021.589518.

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 Creators:
Soto, Maria J., Author
Prabhakar, Pradeep Kumar, Author
Wang, Hsin-Tzu, Author
Backe, Jason, Author
Chapla, Digantkumar, Author
Bartetzko, Max Peter1, Author              
Black, Ian M., Author
Azadi, Parastoo, Author
Peña, Maria J., Author
Pfrengle, Fabian1, Author              
Moremen, Kelley W., Author
Urbanowicz, Breeanna R., Author
Hahn, Michael G., Author
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

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Free keywords: Fucosyltransferase, arabinogalactan protein, AtFUT1, AtFUT4, AtFUT6, GT37, plant cell wall, hydroxyproline-rich glycoprotein
 Abstract: The bulk of plant biomass is comprised of plant cell walls, which are complex polymeric networks, composed of diverse polysaccharides, proteins, polyphenolics, and hydroxyproline-rich glycoproteins (HRGPs). Glycosyltransferases (GTs) work together to synthesize the saccharide components of the plant cell wall. The Arabidopsis thaliana fucosyltransferases (FUTs), AtFUT4, and AtFUT6, are members of the plant-specific GT family 37 (GT37). AtFUT4 and AtFUT6 transfer fucose (Fuc) onto arabinose (Ara) residues of arabinogalactan (AG) proteins (AGPs) and have been postulated to be non-redundant AGP-specific FUTs. AtFUT4 and AtFUT6 were recombinantly expressed in mammalian HEK293 cells and purified for biochemical analysis. We report an updated understanding on the specificities of AtFUT4 and AtFUT6 that are involved in the synthesis of wall localized AGPs. Our findings suggest that they are selective enzymes that can utilize various arabinogalactan (AG)-like and non-AG-like oligosaccharide acceptors, and only require a free, terminal arabinofuranose. We also report with GUS promoter-reporter gene studies that AtFUT4 and AtFUT6 gene expression is sub-localized in different parts of developing A. thaliana roots.

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Language(s): eng - English
 Dates: 2021-02-092021
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.3389/fpls.2021.589518
BibTex Citekey: 10.3389/fpls.2021.589518
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Title: Frontiers in Plant Science
  Abbreviation : Front. Plant Sci.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 12 Sequence Number: 589518 Start / End Page: - Identifier: ISSN: 1664-462X