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  Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142

Polerecky, L., Masuda, T., Eichner, M., Rabouille, S., Vancova, M., Kienhuis, M. V. M., et al. (2021). Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142. Frontiers in Microbiology, 12: 620915. doi:10.3389/fmicb.2021.620915.

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 Creators:
Polerecky, Lubos, Author
Masuda, Takako, Author
Eichner, Meri1, Author           
Rabouille, Sophie, Author
Vancova, Marie, Author
Kienhuis, Michiel V. M., Author
Bernat, Gabor, Author
Bonomi-Barufi, Jose, Author
Campbell, Douglas Andrew, Author
Claquin, Pascal, Author
Cerveny, Jan, Author
Giordano, Mario, Author
Kotabova, Eva, Author
Kromkamp, Jacco, Author
Lombardi, Ana Teresa, Author
Lukes, Martin, Author
Prasil, Ondrej, Author
Stephan, Susanne, Author
Suggett, David, Author
Zavrel, Tomas, Author
Halsey, Kimberly H., Author more..
Affiliations:
1Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481711              

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 Abstract: Unicellular nitrogen fixing cyanobacteria (UCYN) are abundant members of phytoplankton communities in a wide range of marine environments, including those with rapidly changing nitrogen (N) concentrations. We hypothesized that differences in N availability (N-2 vs. combined N) would cause UCYN to shift strategies of intracellular N and C allocation. We used transmission electron microscopy and nanoscale secondary ion mass spectrometry imaging to track assimilation and intracellular allocation of C-13-labeled CO2 and N-15-labeled N-2 or NO3 at different periods across a diel cycle in Cyanothece sp. ATCC 51142. We present new ideas on interpreting these imaging data, including the influences of pre-incubation cellular C and N contents and turnover rates of inclusion bodies. Within cultures growing diazotrophically, distinct subpopulations were detected that fixed N-2 at night or in the morning. Additional significant within-population heterogeneity was likely caused by differences in the relative amounts of N assimilated into cyanophycin from sources external and internal to the cells. Whether growing on N-2 or NO3, cells prioritized cyanophycin synthesis when N assimilation rates were highest. N assimilation in cells growing on NO3 switched from cyanophycin synthesis to protein synthesis, suggesting that once a cyanophycin quota is met, it is bypassed in favor of protein synthesis. Growth on NO3 also revealed that at night, there is a very low level of CO2 assimilation into polysaccharides simultaneous with their catabolism for protein synthesis. This study revealed multiple, detailed mechanisms underlying C and N management in Cyanothece that facilitate its success in dynamic aquatic environments.

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Language(s): eng - English
 Dates: 2021-02-04
 Publication Status: Published online
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000619068700001
DOI: 10.3389/fmicb.2021.620915
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Title: Frontiers in Microbiology
  Abbreviation : Front. Microbiol.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 12 Sequence Number: 620915 Start / End Page: - Identifier: ISSN: 1664-302X
CoNE: https://pure.mpg.de/cone/journals/resource/1664-302X