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  Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

Bokelmann, L., Nickel, O., Maricic, T., Pääbo, S., Meyer, M., Borte, S., et al. (2021). Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP. Nature Communications, 12(1): 1467. doi:10.1038/s41467-021-21627-0.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-18EC-4 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-98E7-5
Genre: Journal Article

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This article is licensed under a Creative CommonsAttribution 4.0 International License, which permits use, sharing,adaptation, distribution and reproduction in any medium or format, as long as you giveappropriate credit to the original author(s) and the source, provide a link to the CreativeCommons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unlessindicated otherwise in a credit line to the material. If material is not included in thearticle’s Creative Commons license and your intended use is not permitted by statutoryregulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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 Creators:
Bokelmann, Lukas1, 2, Author                 
Nickel, Olaf, Author
Maricic, Tomislav2, Author                 
Pääbo, Svante2, Author           
Meyer, Matthias3, Author           
Borte, Stephan, Author
Riesenberg, Stephan2, Author                 
Affiliations:
1The Leipzig School of Human Origins (IMPRS), Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497688              
2Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497672              
3Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_2074332              

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 Abstract: Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.

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Language(s): eng - English
 Dates: 2021-03-05
 Publication Status: Published online
 Pages: -
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-21627-0
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Title: Nature Communications
Source Genre: Journal
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Pages: - Volume / Issue: 12 (1) Sequence Number: 1467 Start / End Page: - Identifier: ISSN: 2041-1723