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  Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

Bokelmann, L., Nickel, O., Maricic, T., Pääbo, S., Meyer, M., Borte, S., et al. (2021). Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP. Nature Communications, 12(1): 1467. doi:10.1038/s41467-021-21627-0.

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 Creators:
Bokelmann, Lukas1, 2, Author                 
Nickel, Olaf, Author
Maricic, Tomislav2, Author                 
Pääbo, Svante2, Author           
Meyer, Matthias3, Author           
Borte, Stephan, Author
Riesenberg, Stephan2, Author                 
Affiliations:
1The Leipzig School of Human Origins (IMPRS), Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497688              
2Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497672              
3Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_2074332              

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 Abstract: Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.

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Language(s): eng - English
 Dates: 2021-03-05
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-21627-0
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Title: Nature Communications
Source Genre: Journal
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Pages: - Volume / Issue: 12 (1) Sequence Number: 1467 Start / End Page: - Identifier: ISSN: 2041-1723