Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

Bokelmann, L., Nickel, O., Maricic, T., Pääbo, S., Meyer, M., Borte, S., et al. (2021). Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP. Nature Communications, 12(1): 1467. doi:10.1038/s41467-021-21627-0.

Item is

 

einblenden: alle

Basisdaten

ausblenden:
Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0008-18EC-4 Versions-Permalink: https://hdl.handle.net/21.11116/0000-000F-4997-5
Genre: Zeitschriftenartikel

Dateien

ausblenden: Dateien
:
Bokelmann_Point-of-care_NatComm_2021.pdf (Verlagsversion), 2MB
Öffnen   Speichern
Datei-Permalink:
https://hdl.handle.net/21.11116/0000-0008-18FA-4
Name:
Bokelmann_Point-of-care_NatComm_2021.pdf
Beschreibung:
-
OA-Status:
Gold
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Öffnen
Copyright Datum:
2021
Copyright Info:
This article is licensed under a Creative CommonsAttribution 4.0 International License, which permits use, sharing,adaptation, distribution and reproduction in any medium or format, as long as you giveappropriate credit to the original author(s) and the source, provide a link to the CreativeCommons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unlessindicated otherwise in a credit line to the material. If material is not included in thearticle’s Creative Commons license and your intended use is not permitted by statutoryregulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Lizenz:
https://creativecommons.org/licenses/by/4.0/
:
Bokelmann_Point-of-care_NatComm_2021_Suppl..pdf (Ergänzendes Material), 4MB
Öffnen   Speichern
Datei-Permalink:
https://hdl.handle.net/21.11116/0000-0008-1900-C
Name:
Bokelmann_Point-of-care_NatComm_2021_Suppl.pdf
Beschreibung:
-
OA-Status:
Keine Angabe
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Öffnen
Copyright Datum:
2021
Copyright Info:
This article is licensed under a Creative CommonsAttribution 4.0 International License, which permits use, sharing,adaptation, distribution and reproduction in any medium or format, as long as you giveappropriate credit to the original author(s) and the source, provide a link to the CreativeCommons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unlessindicated otherwise in a credit line to the material. If material is not included in thearticle’s Creative Commons license and your intended use is not permitted by statutoryregulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Lizenz:
https://creativecommons.org/licenses/by/4.0/

Externe Referenzen

ausblenden:
externe Referenz:
https://www.medrxiv.org/content/10.1101/2020.08.04.20168617v1 (Preprint)
Beschreibung:
Preprint
OA-Status:
Keine Angabe

Urheber

ausblenden:
 Urheber:
Bokelmann, Lukas1, 2, Autor                 
Nickel, Olaf, Autor
Maricic, Tomislav2, Autor                 
Pääbo, Svante2, Autor                 
Meyer, Matthias3, Autor           
Borte, Stephan, Autor
Riesenberg, Stephan2, Autor                 
Affiliations:
1The Leipzig School of Human Origins (IMPRS), Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497688              
2Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497672              
3Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_2074332              

Inhalt

ausblenden:
Schlagwörter: -
 Zusammenfassung: Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.

Details

ausblenden:
Sprache(n): eng - English
 Datum: 2021-03-05
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1038/s41467-021-21627-0
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

ausblenden:
Titel: Nature Communications
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 12 (1) Artikelnummer: 1467 Start- / Endseite: - Identifikator: ISSN: 2041-1723