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  Downregulation of surface sodium pumps by endocytosis during meiotic maturation of Xenopus laevis oocytes

Schmalzing, G., Eckard, P., Kröner, S., & Passow, H. (1990). Downregulation of surface sodium pumps by endocytosis during meiotic maturation of Xenopus laevis oocytes. American Journal of Physiology: Cell Physiology, 258(1), C179-C184. doi:10.1152/ajpcell.1990.258.1.C179.

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 Creators:
Schmalzing, Günther1, Author           
Eckard, Peter1, Author           
Kröner, Silke1, Author           
Passow, Hermann1, Author           
Affiliations:
1Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              

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 Abstract: During meiotic maturation, plasma membranes of Xenopus laevis oocytes completely lose the capacity to transport Na and K and to bind ouabain. To explore whether the downregulation might be due to an internalization of the sodium pump molecules, the intracellular binding of ouabain was determined. Selective permeabilization of the plasma membrane of mature oocytes (eggs) by digitonin almost failed to disclose ouabain binding sites. However, when the eggs were additionally treated with 0.02% sodium dodecyl sulfate (SDS) to permeabilize inner membranes, all sodium pumps present before maturation were recovered. Phosphorylation by [gamma-32P]ATP combined with SDS-polyacrylamide gel electrophoresis (PAGE) and autoradiography showed that sodium pumps were greatly reduced in isolated plasma membranes of eggs. According to sucrose gradient fractionation, maturation induced a shift of sodium pumps from the plasma membrane fraction to membranes of lower buoyant density with a protein composition different from that of the plasma membrane. Endocytosed sodium pumps identified on the sucrose gradient from [3H]ouabain bound to the cell surface before maturation could be phosphorylated with inorganic [32P]phosphate. The findings suggest that downregulation of sodium pumps during maturation is brought about by translocation of surface sodium pumps to an intracellular compartment, presumably endosomes. This contrasts the mechanism of downregulation of Na-dependent cotransport systems, the activities of which are reduced as a consequence of a maturation-induced depolarization of the membrane without a removal of the corresponding transporter from the plasma membrane.

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Language(s): eng - English
 Dates: 1989-06-221989-09-111990-01-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1152/ajpcell.1990.258.1.C179
 Degree: -

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Title: American Journal of Physiology: Cell Physiology
Source Genre: Journal
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Publ. Info: American Physiological Society
Pages: - Volume / Issue: 258 (1) Sequence Number: - Start / End Page: C179 - C184 Identifier: ISSN: 0363-6143
CoNE: https://pure.mpg.de/cone/journals/resource/954925523731