English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Key factors regulating Plasmodium berghei sporozoite survival and transformation revealed by an automated visual assay

Hegge, S., Kudryashev, M., Barniol, L., & Frischknecht, F. (2010). Key factors regulating Plasmodium berghei sporozoite survival and transformation revealed by an automated visual assay. The FASEB Journal, 24(12), 5003-5012. doi:10.1096/fj.10.164814.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hegge, Stephan1, Author
Kudryashev, Misha1, Author              
Barniol, Luis1, Author
Frischknecht, Friedrich1, Author
Affiliations:
1Parasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: lucose; bicarbonate; screening; Apicomplexa; differentiation
 Abstract: Malaria is transmitted to the host when Plasmodium sporozoites are injected by a mosquito vector. Sporozoites eventually enter hepatocytes, where they differentiate into liver-stage parasites. During the first hours after hepatocyte invasion, the crescent-shaped sporozoites transform into spherical intracellular exoerythrocytic parasites. This process, which precedes genome replication, can be mimicked in vitro in the absence of host cells. Here, we developed an automated method to follow transformation and cell death of sporozoites in vitro. This assay provides a rapid tool to test sporozoite survival and to screen for antiparasitic drugs. We found that extracellular bicarbonate and high temperature trigger transformation, whereas physiological serum albumin concentrations and media lacking bicarbonate delayed sporozoite death. Because bicarbonate also triggers ookinete transformation and exflagellation of gametocytes, we suggest that a common molecular mechanism regulates similar aspects of stage conversion in Plasmodium.

Details

show
hide
Language(s): eng - English
 Dates: 2010-06-082010-08-122010-12-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1096/fj.10.164814
PMID: 20798246
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The FASEB Journal
  Other : FASEB J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : The Federation
Pages: - Volume / Issue: 24 (12) Sequence Number: - Start / End Page: 5003 - 5012 Identifier: ISSN: 0892-6638
CoNE: https://pure.mpg.de/cone/journals/resource/954927535970_1