hide
Free keywords:
fluorescence spectroscopy; high‐throughput screening;
microfluidics
microreactors
molecular evolution
Abstract:
Just a drop. In the field of biological research and biotechnology, fast and efficient screening for particular properties of minute quantities down to single‐particle level has become increasingly important. This work describes the implementation of a lab‐on‐chip device to generate small reaction containers, that is, a homodisperse water‐in‐oil emulsion, and cell‐free expression of the green fluorescent protein inside the aqueous droplets. Detection of the autofluorescent protein was performed by confocal fluorescence spectroscopy.
