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  A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells

Scalera, V., Storelli, C., Storelli-Joss, C., Haase, W., & Murer, H. (1980). A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells. Biochemical Journal, 186(1), 177-181. doi:10.1042/bj1860177.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-3100-0 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-3101-F
Genre: Journal Article

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 Creators:
Scalera, Vito1, Author           
Storelli, Carlo1, Author           
Storelli-Joss, Christina1, Author           
Haase, Winfried1, Author           
Murer, Heini1, Author           
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1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

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 Abstract: A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na++ K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.

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Language(s): eng - English
 Dates: 1979-05-171980-01-15
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1042/bj1860177
PMID: 6245639
PMC: PMC1161517
 Degree: -

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Title: Biochemical Journal
Source Genre: Journal
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Publ. Info: London : Published by Portland Press on behalf of the Biochemical Society.
Pages: - Volume / Issue: 186 (1) Sequence Number: - Start / End Page: 177 - 181 Identifier: ISSN: 0264-6021
CoNE: https://pure.mpg.de/cone/journals/resource/110992357308158