English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-performance liquid chromatographic purification of extremely hydrophobic peptides: transmembrane segments

Bollhagen, R., Schmiedberger, M., & Grell, E. (1995). High-performance liquid chromatographic purification of extremely hydrophobic peptides: transmembrane segments. Journal of Chromatography A, 711(1), 181-186. doi:10.1016/0021-9673(95)00056-s.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bollhagen, Ralf1, Author           
Schmiedberger, Monika1, Author           
Grell, Ernst1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

Content

show
hide
Free keywords: -
 Abstract: Transmembrane peptides of integral membrane proteins often exhibit extremely high hydrophobicity. Therefore, the solubility of such peptides in solvents commonly used in HPLC is usually very low and the interaction with generally applied stationary phases such as silica gel or C18 reversed phases appears to be extremely strong, which makes the characterization and purification of these peptides difficult. The analytical characterization and preparative separation of the synthesized M1 transmembrane sequence of the inhibitory glycine receptor Mr 48,000 subunit and some of its fragments is shown. M1 and its larger fragments could be dissolved in a dichloromethane-hexafluoro-2-propanol mixture containing a trace amount of pyridine for their separation on a C4 phase by employing linear two-component gradients of formic acid-2-propanol and formic acid-water with ratios up to 4:1 (v/v). Conditions to avoid formylation of the peptides are indicated.

Details

show
hide
Language(s): eng - English
 Dates: 2000-04-071995-09-08
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0021-9673(95)00056-s
PMID: 7496489
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Chromatography A
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 711 (1) Sequence Number: - Start / End Page: 181 - 186 Identifier: ISSN: 0021-9673
CoNE: https://pure.mpg.de/cone/journals/resource/954925527837_1