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  Exchangeable oxygens in the vicinity of the molybdenum center of the high-pH form of sulfite oxidase and sulfite dehydrogenase

Astashkin, A. V., Klein, E. L., Ganyushin, D., Johnson-Winters, K., Neese, F., Kappler, U., et al. (2009). Exchangeable oxygens in the vicinity of the molybdenum center of the high-pH form of sulfite oxidase and sulfite dehydrogenase. Physical Chemistry Chemical Physics, 11(31), 6733-6742. doi:10.1039/B907029J.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-319D-0 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-319E-F
Genre: Journal Article

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 Creators:
Astashkin, Andrei V.1, Author
Klein, Eric L.1, Author
Ganyushin, Dmitry2, Author
Johnson-Winters, Kayunta1, Author
Neese, Frank2, Author           
Kappler, Ulrike3, Author
Enemark, John H.1, Author
Affiliations:
1Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA, ou_persistent22              
2Lehrstuhl für Theoretische Chemie, Institut für Physikalische und Theoretische Chemie, Universität Bonn, Wegelerstrasse 12, D-53115 Bonn, Germany, ou_persistent22              
3Centre for Metals in Biology, University of Queensland, St. Lucia, Queensland 4072, Australia, ou_persistent22              

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 Abstract: The electron spin echo envelope modulation (ESEEM) investigation of the high-pH (hpH) form of sulfite oxidase (SO) and sulfite dehydrogenase (SDH) prepared in buffer enriched with H217O reveals the presence of three types of exchangeable oxygen atoms at the molybdenum center. Two of these oxygen atoms belong to the equatorial OH ligand and the axial oxo ligand, and are characterized by 17O hyperfine interaction (hfi) constants of about 37 MHz and 6 MHz, respectively. The third oxygen has an isotropic hfi constant of 3–4 MHz and likely belongs to a hydroxyl moiety hydrogen-bonded to the equatorial OH ligand. This exchangeable oxygen atom is not observed in the ESEEM spectra of the Y236F mutant of SDH, where the active site tyrosine has been replaced by phenylalanine.

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Language(s): eng - English
 Dates: 2009-04-062009-07-132009-08-21
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1039/B907029J
 Degree: -

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Title: Physical Chemistry Chemical Physics
  Abbreviation : Phys. Chem. Chem. Phys.
Source Genre: Journal
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Publ. Info: Cambridge, England : Royal Society of Chemistry
Pages: - Volume / Issue: 11 (31) Sequence Number: - Start / End Page: 6733 - 6742 Identifier: ISSN: 1463-9076
CoNE: https://pure.mpg.de/cone/journals/resource/954925272413_1