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  Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles

Fu, M., Franquelim, H. G., Kretschmer, S., & Schwille, P. (2021). Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles. Angewandte Chemie International Edition, 60(12), 6496-6502. doi:10.1002/anie.202015184.

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© 2020 The Authors. Open access funding enabled and organized by Projekt DEAL.
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 Creators:
Fu, Meifang1, Author              
Franquelim, Henri G.1, Author              
Kretschmer, Simon1, Author              
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: GIANT VESICLES; CELL-DIVISION; SELF-ORGANIZATION; BINDING; DYNAMICS; FISSION; AGGREGATION; STIMULATION; CURVATURE; ATPASEChemistry; artificial cells; membrane; reaction-diffusion; self-organization; synthetic biology;
 Abstract: The MinDE proteins from E. coli have received great attention as a paradigmatic biological pattern-forming system. Recently, it has surfaced that these proteins do not only generate oscillating concentration gradients driven by ATP hydrolysis, but that they can reversibly deform giant vesicles. In order to explore the potential of Min proteins to actually perform mechanical work, we introduce a new model membrane system, flat vesicle stacks on top of a supported lipid bilayer. MinDE oscillations can repeatedly deform these flat vesicles into tubules and promote progressive membrane spreading through membrane adhesion. Dependent on membrane and buffer compositions, Min oscillations further induce robust bud formation. Altogether, we demonstrate that under specific conditions, MinDE self-organization can result in work performed on biomimetic systems and achieve a straightforward mechanochemical coupling between the MinDE biochemical reaction cycle and membrane transformation.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: We thank MPI‐B Core Facility for assistance in protein purification.
 Rev. Type: -
 Identifiers: ISI: 000611929200001
DOI: 10.1002/anie.202015184
 Degree: -

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Project name : Grant Number: Ref 3.5-1207791-CHN-HFST-P
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Funding organization : Alexander von Humboldt-Stiftung
Project name : Grant Numbers: SFB1032, SFB863
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Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 60 (12) Sequence Number: - Start / End Page: 6496 - 6502 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851