Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  DNA sequence encodes the position of DNA supercoils

Kim, S. H., Ganji, M., Kim, E., Kim, E., van der Torre, J., Abbondanzieri, E., et al. (2018). DNA sequence encodes the position of DNA supercoils. eLife, 7: e36557. doi:10.7554/eLife.36557.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Kim, Sung Hyun1, Autor
Ganji, Mahipal1, Autor
Kim, Eugene, Autor
Kim, Eugene2, Autor                 
van der Torre, Jaco1, Autor
Abbondanzieri, Elio1, Autor
Dekker, Cees1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, Netherlands, ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Base Sequence, Biotin, C. elegans, Carbocyanines, chromosome organization, chromosomes, D. melanogaster, DNA structure, DNA Supercoil, DNA, Bacterial, DNA, Superhelical, E. coli, fluorescence, Fluorescent Dyes, gene expression, Microscopy, Fluorescence, Organic Chemicals, physics of living systems, Plasmids, plectoneme, Polymerase Chain Reaction, Promoter Regions, Genetic, S. cerevisiae, single molecule, Streptavidin
 Zusammenfassung: The three-dimensional organization of DNA is increasingly understood to play a decisive role in vital cellular processes. Many studies focus on the role of DNA-packaging proteins, crowding, and confinement in arranging chromatin, but structural information might also be directly encoded in bare DNA itself. Here, we visualize plectonemes (extended intertwined DNA structures formed upon supercoiling) on individual DNA molecules. Remarkably, our experiments show that the DNA sequence directly encodes the structure of supercoiled DNA by pinning plectonemes at specific sequences. We develop a physical model that predicts that sequence-dependent intrinsic curvature is the key determinant of pinning strength and demonstrate this simple model provides very good agreement with the data. Analysis of several prokaryotic genomes indicates that plectonemes localize directly upstream of promoters, which we experimentally confirm for selected promotor sequences. Our findings reveal a hidden code in the genome that helps to spatially organize the chromosomal DNA.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2018-03-102018-12-062018-12-07
 Publikationsstatus: Online veröffentlicht
 Seiten: 23
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.7554/eLife.36557
BibTex Citekey: kim_dna_2018
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: eLife
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Cambridge : eLife Sciences Publications
Seiten: - Band / Heft: 7 Artikelnummer: e36557 Start- / Endseite: - Identifikator: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X