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  Identification of a myometrial oxytocin-receptor protein

Fahrenholz, F., Hackenberg, M., & Müller, M. (1988). Identification of a myometrial oxytocin-receptor protein. European Journal of Biochemistry, 174(1), 81-85. doi:10.1111/j.1432-1033.1988.tb14065.x.

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 Creators:
Fahrenholz, Falk1, Author           
Hackenberg, Mario1, Author           
Müller, Michael1, Author           
Affiliations:
1Department of Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_3264819              

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 Abstract: The specific binding of [3H]oxytoxin to uterine membrane preparations derived from different species at late pregnancy was examined. The highest receptor density (bmax value) was found in membranes derived from the myometria of guinea pigs between day 60 post-conception (bm = 3.6 ± 0.1 pmol/mg) and day 65 (bmax = 4.4 ± 0.1 pmol/mg). The similarity of Kd values for oxytocin binding (Kd = 2.6 ± 0.2 nM) and for vasopressin binding (Kd = 2.1 ± 0.4 nM) to the same membranes derived from a guinea pig myometrium indicate a homogeneous population of high-affinity binding sites which do not discriminate between these two hormones. Competitive binding experiments with specific oxytocin agonists containing either sarcosine or N-methylalanine in the place of Pro7 demonstrated that these myometrial receptors have the pharmacological properties of oxytocin receptors. The analogue of 1-deamino-[8-lysine]vasopressin containing a photoreactive azidophenylamidino group at the sidechain of Lys8 retained roughly the same receptor affinity as oxytocin. In photoaffinity labelling experiments with the tritium-labelled analogue a membrane protein from guinea pig myometrium with an apparent relative molecular mass Mr of 78,000 ± 5000 (n = 13) was preferentially labelled. The labelling of this protein was completely suppressed by a 100-fold molar excess of either oxytocin, or [Sar7]oxytocin or [Thr4, Sar7]oxytocin, but not by other peptide hormones. These results provide evidence that the labelled 78,000-Mr protein is a myometrial oxytocin-receptor protein.

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Language(s): eng - English
 Dates: 1988-01-251987-11-262005-03-031988-05-16
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1111/j.1432-1033.1988.tb14065.x
PMID: 2836202
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Title: European Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 174 (1) Sequence Number: - Start / End Page: 81 - 85 Identifier: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040