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  Expression of a Bacterial Trehalose-6-phosphate Synthase otsA Increases Oil Accumulation in Plant Seeds and Vegetative Tissues

Zhai, Z., Keereetaweep, J., Liu, H., Feil, R., Lunn, J. E., & Shanklin, J. (2021). Expression of a Bacterial Trehalose-6-phosphate Synthase otsA Increases Oil Accumulation in Plant Seeds and Vegetative Tissues. Frontiers in Plant Science, 12: 656962. doi:10.3389/fpls.2021.656962.

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Zhai, Zhiyang1, Author
Keereetaweep, Jantana1, Author
Liu, Hui1, Author
Feil, R.2, Author              
Lunn, J. E.2, Author              
Shanklin, John1, Author
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1External Organizations, ou_persistent22              
2System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              

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 Abstract: We previously demonstrated that exogenous trehalose 6-phosphate (T6P) treatment stabilized WRINKLED1 (WRI1), a master transcriptional regulator of fatty acid (FA) synthesis and increased total FA content in Brassica napus (B. napus) embryo suspension cell culture. Here, we explore Arabidopsis lines heterologously expressing the Escherichia coli T6P synthase (otsA) or T6P phosphatase (otsB) to refine our understanding regarding the role of T6P in regulating fatty acid synthesis both in seeds and vegetative tissues. Arabidopsis 35S:otsA transgenic seeds showed an increase of 13 in fatty acid content compared to those of wild type (WT), while seeds of 35:otsB transgenic seeds showed a reduction of 12 in fatty acid content compared to WT. Expression of otsB significantly reduced the level of WRI1 and expression of its target genes in developing seeds. Like Arabidopsis seeds constitutively expressing otsA, transient expression of otsA in Nicotiana benthamiana leaves resulted in strongly elevated levels of T6P. This was accompanied by an increase of 29 in de novo fatty acid synthesis rate, a 2.3-fold increase in triacylglycerol (TAG) and a 20 increase in total fatty acid content relative to empty vector (EV) controls. Taken together, these data support the heterologous expression of otsA as an approach to increasing TAG accumulation in plant seeds and vegetative tissues.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.3389/fpls.2021.656962
BibTex Citekey: 10.3389/fpls.2021.656962
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Title: Frontiers in Plant Science
  Abbreviation : Front. Plant Sci.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 12 Sequence Number: 656962 Start / End Page: - Identifier: ISSN: 1664-462X
CoNE: https://pure.mpg.de/cone/journals/resource/1664462X