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  EPR experiments to elucidate the structure of the ready and unready states of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F

van Gastel, M., Fichtner, C., Neese, F., & Lubitz, W. (2005). EPR experiments to elucidate the structure of the ready and unready states of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F. Biochemical Society Transactions, 33(1), 7-11. doi:10.1042/BST0330007.

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 Creators:
van Gastel, M.1, Author              
Fichtner, C.1, Author              
Neese, F.2, Author              
Lubitz, W.1, Author              
Affiliations:
1Research Department Lubitz, Max Planck Institute for Bioinorganic Chemistry, Max Planck Society, ou_3023878              
2Research Department Wieghardt, Max Planck Institute for Bioinorganic Chemistry, Max Planck Society, ou_3023881              

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Free keywords: Desulfovibrio vulgaris Miyazaki F; density functional theory (DFT); electron nuclear double resonance (ENDOR); EPR; hydrogenase; hyperfine sublevel correlation (HYSCORE)
 Abstract: Isolation and purification of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F under aerobic conditions leads to a mixture of two states, Ni-A (unready) and Ni-B (ready). The two states are distinguished by different activation times and different EPR spectra. HYSCORE and ENDOR data and DFT calculations show that both states have an exchangeable proton, albeit with a different 1H hyperfine coupling. This proton is assigned to the bridging ligand between Ni and Fe. For Ni-B, a hydroxo ligand is found. For Ni-A, either a hydroxo in a different orientation or a hydroperoxo-bridging ligand is present.

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Language(s): eng - English
 Dates: 2004-10-222005-02-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1042/BST0330007
 Degree: -

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Title: Biochemical Society Transactions
  Abbreviation : Biochem. Soc. Trans.
Source Genre: Journal
 Creator(s):
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Publ. Info: London, UK : Portland Press
Pages: - Volume / Issue: 33 (1) Sequence Number: - Start / End Page: 7 - 11 Identifier: ISSN: 0300-5127
CoNE: https://pure.mpg.de/cone/journals/resource/954925507337