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  Acetazolamide inhibition of basolateral base exit in rabbit renal proximal tubule S2 segment

Seki, G., & Frömter, E. (1992). Acetazolamide inhibition of basolateral base exit in rabbit renal proximal tubule S2 segment. Pflügers Archiv: European Journal of Physiology, 422, 60-65. doi:10.1007/BF00381514.

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 Creators:
Seki, G.1, 2, Author           
Frömter, Eberhard2, Author
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              
2Zentrum der Physiologie, Johann Wolfgang Goethe-Universität, W-6000, Frankfurt am Main, Federal Republic of Germany, ou_persistent22              

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Free keywords: Renal proximal tubule; S2 segment; Rheogenic Na+-HCO3 cotransport; Cl/HCO3 exchange; Carbonic anhydrase inhibitor
 Abstract: The influence of the carbonic anhydrase inhibitor acetazolamide (ACZ) was investigated on HCO3 transport mechanisms in the basolateral cell membrane of rabbit renal proximal tubule. Experiments were performed on isolated S2 segments using double-barrelled microelectrodes to measure cell membrane potential (Vb) and cell pH (pHi) during step changes in bath perfusate ion concentrations. Peritubular application of ACZ (1 mmol/l) reduced the initial Vb response to 10∶1 reduction of bath HCO3 concentration only slightly, from +53.8±4.2 mV to+49.1±0.3 mV (n=5), but caused an intermittent overshooting repolarization in the secondary Vb response. In conjunction with these effects it left the initial pHi response virtually unchanged but induced a secondary slow acidification. These observation indicate that — under the present experimental conditions — ACZ does not block the Na+-HCO3 cotransporter but acts via inhibition of cytosolic carbonic anhydrase. This was confirmed by studying the effect of elevated intracellular HCO3 concentrations under reduced flux conditions and by comparing the concentration dependence of the Vb response with the inhibition kinetics of cytosolic carbonic anhydrase. In contrast, peritubular ACZ inhibited Na+-independent Cl−/HCO3 exchange in the basolateral cell membrane of S2 segments directly in a similar way to that described in the preceding publication for S3 segments.

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Language(s): eng - English
 Dates: 1992-07-281991-12-311992-07-281992-10-01
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/BF00381514
PMID: 1437525
 Degree: -

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Title: Pflügers Archiv: European Journal of Physiology
  Other : Pflügers Arch. Europ. J. Physiol.
Source Genre: Journal
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Publ. Info: Heidelberg : Springer-Verlag
Pages: - Volume / Issue: 422 Sequence Number: - Start / End Page: 60 - 65 Identifier: ISSN: 0031-6768
CoNE: https://pure.mpg.de/cone/journals/resource/954925432380