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  Structural changes on binding FAD and FAD-analogous and on site-directed mutagenesis of glutathione reductase

Schulz, G. E., & Ermler, U. (1991). Structural changes on binding FAD and FAD-analogous and on site-directed mutagenesis of glutathione reductase. In B. Curti (Ed.), Flavins and Flavoproteins 1990 (pp. 505-512). Berlin, New York: Walter de Gruyter. doi:10.1515/9783110855425-098.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-37ED-0 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-A8D0-C
Genre: Conference Paper

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 Creators:
Schulz, Georg E.1, Author
Ermler, Ulrich1, Author                 
Affiliations:
1Institut für Organische Chemie und Biochemie, Universität Freiburg, D‐7800 Freiburg im Bresgau, Federal Republic of Germany, ou_persistent22              

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 Abstract: Glutathione reductase from human erythrocytes was the first flavoenzyme studied in great structural detail. It belongs to a sizable family of flavoenzymes. Several binding studies were pursued at medium resolution. At high resolution 4 intermediate states were analyzed and refined, yielding a detailed view of the catalytic cycle. The apo-enzyme is rather stable and could be crystallized. It can be used for reconstituting the holo-enzyme with FAD and with a number of FAD-analogues. The changes after removal of FAD and after reconstitution with FAD-analogues are here discussed. Glutathione reductase from E.coli has been analyzed by site-directed mutagenesis as guided by the known homologous structure of the human enzyme (53% identity, Ref.26). Most conspicuous was the change from NADPH to NADH by seven individual point mutations. For analyzing these mutants in detail we solved the structure of the E.coli enzyme.

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Language(s): eng - English
 Dates: 2019-07-221991-04-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1515/9783110855425-098
 Degree: -

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Title: Proceedings of the Tenth International Symposium on Flavins and Flavoproteins
Place of Event: Como, Italy
Start-/End Date: 1990-07-15 - 1990-07-20

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Title: Flavins and Flavoproteins 1990
Source Genre: Proceedings
 Creator(s):
Curti, Bruno1, Editor
Ronchi, Severino2, Author
Zanetti, G.1, Author
Affiliations:
1 Dipartimento di Fisiologia e Biochimica Generali, Università Statale di Milano, Via Celoria 26, 1-20133 Milano, Italia , ou_persistent22            
2 Istituto di Fisiologia Veterinaria e Biochimica, Università Statale di Milano, Via Celoria 26, 1-20133 Milano, Italia, ou_persistent22            
Publ. Info: Berlin, New York : Walter de Gruyter
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 505 - 512 Identifier: DOI: 10.1515/9783110855425
ISBN: 9783110855425
ISBN: 3-11-012373-8