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  Voltage-dependent inhibition of the Na+,K+ pump by tetraethylammonium

Eckstein-Ludwig, U., Rettinger, J., Vasilets, L. A., & Schwarz, W. (1998). Voltage-dependent inhibition of the Na+,K+ pump by tetraethylammonium. Biochimica et Biophysica Acta-Biomembranes, 1372(2), 289-300. doi:10.1016/S0005-2736(98)00066-2.

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 Creators:
Eckstein-Ludwig, Ursula1, Author           
Rettinger, Jürgen1, 2, Author           
Vasilets, Larisa A.1, 3, Author           
Schwarz, Wolfgang1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Pharmakologisches Institut, Biozentrum, Johann Wolfgang Goethe-Universität, D-60439 Frankfurt, Germany, ou_persistent22              
3Institute of Chemical Physics, Chernogolovka, Moscow Region, Russia, ou_persistent22              

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Free keywords: TEA; Na+,K+-ATPase; Oocyte; Pump current; Ouabain; (Xenopus)
 Abstract: Tetraethylammonium (TEA+) is an effective inhibitor of a variety of K+ channels, and has been widely used to reduce K+-sensitive background conductances in electrophysiological investigations of the Na+,K+-ATPase. Here we demonstrate by combination of two-electrode voltage clamp (TEVC) and giant patch clamp of Xenopus oocytes, and measurements of the activity of purified ATPase of pig kidney that TEA+ directly inhibits the Na+,K+-ATPase from the outside. The KI value in TEVC experiments at 0 mV is about 10 mM increasing with more negative potentials. A similar voltage-dependent inhibition by TEA+ was observed in the excised membrane patches except that the apparent KI value at 0 mV is about 100 mM, a value nearly identical to that found for inhibition of purified kidney ATPase. The voltage-dependent inhibition can be described by an effective valency of 0.39 and is attributed to an interference with the voltage-dependent binding of K+ at an external access channel. The apparent dielectric length of the access channel for K+ is not affected by TEA+.

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Language(s): eng - English
 Dates: 1998-04-241998-04-141998-11-151998-07-17
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0005-2736(98)00066-2
PMID: 9675315
 Degree: -

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Title: Biochimica et Biophysica Acta-Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1372 (2) Sequence Number: - Start / End Page: 289 - 300 Identifier: ISSN: 0005-2736
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702