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  The Na+,K+-ATPase carrying the carboxy-terminal Ca2+/calmodulin binding domain of the Ca2+ pump has 2Na+,2K+ stoichiometry and lost charge movement in Na+/Na+ exchange

Yoshimura, S. H., Vasilets, L. A., Ishii, T., Takeyasu, K., & Schwarz, W. (1998). The Na+,K+-ATPase carrying the carboxy-terminal Ca2+/calmodulin binding domain of the Ca2+ pump has 2Na+,2K+ stoichiometry and lost charge movement in Na+/Na+ exchange. FEBS Letters, 425(1), 71-74. doi:10.1016/s0014-5793(98)00202-6.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0008-392A-A 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0008-392B-9
資料種別: 学術論文

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 作成者:
Yoshimura, Shige H.1, 2, 著者           
Vasilets, Larisa A.1, 3, 著者           
Ishii, Toshiaki4, 著者
Takeyasu, Kunio2, 著者
Schwarz, Wolfgang1, 著者           
所属:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Faculty of Integrated Human Studies, Kyoto University, Kyoto 606-01, Japan, ou_persistent22              
3Institute of Chemical Physics, Russian Academy of Sciences, 142432 Chernogolovka, Russia, ou_persistent22              
4Research Institute for Advances in Science and Technology, University of Osaka Prefecture, Osaka 591, Japan, ou_persistent22              

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キーワード: Ion transport stoichiometry; Na+,K+-ATPase chimera; Ca2+/calmodulin domain
 要旨: An altered ion-transport stoichiometry from 3Na+,2K+ to 2Na+,2K+ is observed in a chimeric Na+,K+ATPase, which carries the Ca2+/calmodulin binding domain (CBD) of the plasma membrane Ca2+-ATPase at its carboxy-terminus [Zhao et al., FEBS Lett. 408 (1997) 271-2751. The ouabain-resistant mutant of this chimera (ORalpha1-CBD) was constructed to further investigate the effect of the CBD on ion-transport properties. The ORalpha1-CBD still shows the 2Na+,2K+ stoichiometry. The loss of electrogenicity is accompanied by the disappearance of transient charge movements in the Na+/Na+ exchange mode. We conclude that the binding of the third Na+ ion, but not of the two others, in 3Na+,2K+ transport mode apparently senses the electric field, and that the voltage-dependent Na+ binding is likely to be lost in the chimera with CBD.

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言語: eng - English
 日付: 1998-01-281998-06-241998-03-20
 出版の状態: 出版
 ページ: 4
 出版情報: -
 目次: -
 査読: 査読あり
 識別子(DOI, ISBNなど): DOI: 10.1016/s0014-5793(98)00202-6
PMID: 9541009
 学位: -

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出版物 1

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出版物名: FEBS Letters
  その他 : FEBS Lett.
種別: 学術雑誌
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出版社, 出版地: Amsterdam : Elsevier
ページ: - 巻号: 425 (1) 通巻号: - 開始・終了ページ: 71 - 74 識別子(ISBN, ISSN, DOIなど): ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501