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  The Na+,K+-ATPase carrying the carboxy-terminal Ca2+/calmodulin binding domain of the Ca2+ pump has 2Na+,2K+ stoichiometry and lost charge movement in Na+/Na+ exchange

Yoshimura, S. H., Vasilets, L. A., Ishii, T., Takeyasu, K., & Schwarz, W. (1998). The Na+,K+-ATPase carrying the carboxy-terminal Ca2+/calmodulin binding domain of the Ca2+ pump has 2Na+,2K+ stoichiometry and lost charge movement in Na+/Na+ exchange. FEBS Letters, 425(1), 71-74. doi:10.1016/s0014-5793(98)00202-6.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-392A-A Version Permalink: https://hdl.handle.net/21.11116/0000-0008-392B-9
Genre: Journal Article

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 Creators:
Yoshimura, Shige H.1, 2, Author           
Vasilets, Larisa A.1, 3, Author           
Ishii, Toshiaki4, Author
Takeyasu, Kunio2, Author
Schwarz, Wolfgang1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Faculty of Integrated Human Studies, Kyoto University, Kyoto 606-01, Japan, ou_persistent22              
3Institute of Chemical Physics, Russian Academy of Sciences, 142432 Chernogolovka, Russia, ou_persistent22              
4Research Institute for Advances in Science and Technology, University of Osaka Prefecture, Osaka 591, Japan, ou_persistent22              

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Free keywords: Ion transport stoichiometry; Na+,K+-ATPase chimera; Ca2+/calmodulin domain
 Abstract: An altered ion-transport stoichiometry from 3Na+,2K+ to 2Na+,2K+ is observed in a chimeric Na+,K+ATPase, which carries the Ca2+/calmodulin binding domain (CBD) of the plasma membrane Ca2+-ATPase at its carboxy-terminus [Zhao et al., FEBS Lett. 408 (1997) 271-2751. The ouabain-resistant mutant of this chimera (ORalpha1-CBD) was constructed to further investigate the effect of the CBD on ion-transport properties. The ORalpha1-CBD still shows the 2Na+,2K+ stoichiometry. The loss of electrogenicity is accompanied by the disappearance of transient charge movements in the Na+/Na+ exchange mode. We conclude that the binding of the third Na+ ion, but not of the two others, in 3Na+,2K+ transport mode apparently senses the electric field, and that the voltage-dependent Na+ binding is likely to be lost in the chimera with CBD.

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Language(s): eng - English
 Dates: 1998-01-281998-06-241998-03-20
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/s0014-5793(98)00202-6
PMID: 9541009
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 425 (1) Sequence Number: - Start / End Page: 71 - 74 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501