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  Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet

Grünbein, M. L., Gorel, A., Foucar, L., Carbajo, S., Colocho, W., Gilevich, S., et al. (2021). Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet. Nature Communications, 12(1): 1672, pp. 1-11. doi:10.1038/s41467-021-21819-8.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-3975-5 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-E113-3
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 Creators:
Grünbein, Marie Luise1, Author           
Gorel, Alexander1, Author           
Foucar, Lutz1, Author           
Carbajo, Sergio, Author
Colocho, William, Author
Gilevich, Sasha, Author
Hartmann, Elisabeth1, Author           
Hilpert, Mario1, Author           
Hunter, Mark, Author
Kloos, Marco1, Author           
Koglin, Jason E., Author
Lane, Thomas J., Author
Lewandowski, Jim, Author
Lutman, Alberto, Author
Nass, Karol1, Author           
Nass Kovács, Gabriela1, Author           
Roome, Christopher M.1, Author           
Sheppard, John, Author
Shoeman, Robert L.1, Author           
Stricker, Miriam1, Author           
van Driel, Tim, AuthorVetter, Sharon, AuthorDoak, R. Bruce1, Author           Boutet, Sébastien, AuthorAquila, Andrew, AuthorDecker, Franz Josef, AuthorBarends, Thomas R. M.1, Author           Stan, Claudiu Andrei, AuthorSchlichting, Ilme1, Author            more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment employing haemoglobin microcrystals transported via a liquid jet into the XFEL beam. Diffraction data were collected using a shock-wave-free single-pulse scheme as well as the dual-pulse pump-probe scheme. The latter, relative to the former, reveals significant degradation of crystal hit rate, diffraction resolution and data quality. Crystal structures extracted from the two data sets also differ. Since our pump-probe attributes were chosen to emulate EuXFEL operation at its 4.5 MHz maximum pulse rate, this prompts concern about such data collection.

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Language(s): eng - English
 Dates: 2020-10-132021-02-152021-03-15
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-21819-8
URI: https://pubmed.ncbi.nlm.nih.gov/33723266/
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 (1) Sequence Number: 1672 Start / End Page: 1 - 11 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723