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  Isolation of renal brush-border membrane vesicles by a low-speed centrifugation; effect of sex hormones on Na+-H+ exchange in rat and mouse kidney

Mačković, M., Zimolo, Z., Burckhardt, G., & Sabolić, I. (1986). Isolation of renal brush-border membrane vesicles by a low-speed centrifugation; effect of sex hormones on Na+-H+ exchange in rat and mouse kidney. Biochimica et Biophysica Acta-Biomembranes, 862(1), 141-152. doi:10.1016/0005-2736(86)90478-5.

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 Creators:
Mačković, M.1, Author
Zimolo, Z.1, Author
Burckhardt, Gerhard2, Author           
Sabolić, Ivan1, Author
Affiliations:
1Department of Physiology, Faculty of Medicine, University of Zagreb, Zagreb Yugoslavia, ou_persistent22              
2Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

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Free keywords: Na+-H+ exchange; Acridine orange; Steroid hormone; Membrane vesicle; (Mouse kidney)
 Abstract: Na+-H+ exchange in rat and mouse renal brush-border membrane vesicles was studied by fluorescence quenching of the ΔpH indicator, acridine orange. Brush-border membrane vesicles were isolated by a modified Mg/EGTA-precipitation method at low speed centrifugation (8000 × g). The enzymatic characteristics of these membrane vesicles were similar to those obtained by the original high-speed centrifugation method (Biber et al. (1981) Biochim. Biophys. Acta, 647, 169–176). The rates of Na+-H+ exchange in renal brush-border membrane vesicles from male and female rats were similar. Neither ovariectomy nor treatment of ovariectomized rats with estradiol or testosterone changed the activity of Na+-H+ exchanger. The rates of Na+-H+ exchange in the mouse were smaller than in the rat indicating the existence of species differences. Na+-H+ exchange in mouse renal brush-border membranes exhibit strong sex differences, the rates in the male being higher than in the female. Castration of male mice led to a decrease in Na+-H+ exchanges to values found in females. Treatment of castrated mice with estradiol had no effect. In contrast, treatment with testosterone increased the rat of the exchanger by more than 100%. The effect of testosterone was restricted to the Vmax of the Na+-H+ exchanger, whereas the apparent Km for Na+ remained unchanged. Na+-dependent d-glucose transport in mouse renal luminal membranes exhibited also sex differences due to the potent stimulatory effect of testosterone. Therefore, Na+-H+ exchange and Na+-dependent d-glucose transport in the mouse kidney are under control of androgen hormones. This effect could be in close connection with the wellknown renotropic action of androgens in the mouse.

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Language(s): eng - English
 Dates: 1986-07-042002-12-131986-11-06
 Publication Status: Issued
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0005-2736(86)90478-5
PMID: 3768361
 Degree: -

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Title: Biochimica et Biophysica Acta-Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 862 (1) Sequence Number: - Start / End Page: 141 - 152 Identifier: ISSN: 0005-2736
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702