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Free keywords:
Band 3 protein; Sulfate transport; Reconstitution; Turnover number; (Erythrocyte membrane)
Abstract:
The anion transport system of the human erythrocyte membrane was reconstituted in unilamellar phosphatidylcholine vesicles, and a vesicle subpopulation of a narrow size distribution was isolated from the sample by gel filtration. In this subpopulation, the turnover number of the transport protein (the band 3 protein) for sulfate transport was determined. It was found that, in the reconstituted system, the protein transports sulfate 5–10‐times faster than in the human erythrocyte membrane.