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  Microwave-assisted automated glycan assembly

Danglad-Flores, J. A., Leichnitz, S., Sletten, E. T., Joseph, A. A. S., Bienert, K., Le Mai Hoang, K., et al. (2021). Microwave-assisted automated glycan assembly. Journal of the American Chemical Society, 143(23), 8893-8901. doi:10.1021/jacs.1c03851.

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 Creators:
Danglad-Flores, José Angél1, Author              
Leichnitz, Sabrina2, Author              
Sletten, Eric T.1, Author              
Joseph, Abragam A. S.1, Author              
Bienert, Klaus3, Author              
Le Mai Hoang, Kim1, Author              
Seeberger, Peter H.1, Author              
Affiliations:
1Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863306              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              
3Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863284              

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Free keywords: solid phase chemistry; carbohydrate; automated synthesis; glycochemistry; glycobiology; glycosylation
 Abstract: Automated synthesis of DNA, RNA, and peptides provides quickly and reliably important tools for biomedical research. Automated glycan assembly (AGA) is significantly more challenging as highly branched carbohydrates require strict regio- and stereocontrol during synthesis. A new AGA synthesizer enables rapid temperature adjustment from -40 °C to +100 °C to control glycosylations at low temperature and accelerates capping, protecting group removal, and glycan modifications by using elevated temperatures. Thereby, the temporary protecting group portfolio is extended from two to four orthogonal groups that give rise to oligosaccharides with up to four branches. In addition, sulfated glycans and unprotected glycans can be prepared. The new design reduces the typical coupling cycles from 100 min to 60 min while expanding the range of accessible glycans. The instrument drastically shorten and generalizes the synthesis of carbohydrates for use in biomedical and material science.

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Language(s): eng - English
 Dates: 2021-06-012021
 Publication Status: Published in print
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Title: Journal of the American Chemical Society
  Other : JACS
  Abbreviation : J. Am. Chem. Soc.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 143 (23) Sequence Number: - Start / End Page: 8893 - 8901 Identifier: ISSN: 0002-7863

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Title: ChemRxiv : the preprint server for chemistry
Source Genre: Journal
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Publ. Info: Washington, Frankfurt am Main, Cambridge : ACS, GDCh, RSC, et al.
Pages: - Volume / Issue: - Sequence Number: 14292476 Start / End Page: - Identifier: ZDB: 2949094-7