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  A breakthrough in protein engineering of a glycosyltransferase

Reetz, M. T. (2021). A breakthrough in protein engineering of a glycosyltransferase. Green Synthesis and Catalysis, 2(1), 4-5. doi:10.1016/j.gresc.2021.01.007.

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 Creators:
Reetz, Manfred T.1, 2, Author           
Affiliations:
1Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              
2Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China, ou_persistent22              

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Free keywords: Rational design; FRISM; Regioselectivity; Glycosyltransferase
 Abstract: An intriguing advance in the rational protein engineering of a newly identified glycosyltransferase from Siraitia grosvenorii (UGT74AC2) has been accomplished by Zhoutong Sun, Yuanxia Sun and coworkers for the targeted pharmaceutically significant glucosylation of the polyhydroxy substrate silybin. Using an advanced version of Focused Rational Iterative Site-specific Mutagenesis (FRISM), they were able to evolve mutants that allow the formation of the 3-OH, 7-OH and the 3,7-O-diglycoside products with 94%, > 99% and > 99% regioselectivity, respectively. This can be viewed as a breakthrough because the wildtype UGT74AC2 delivers a difficult to separate mixture of products in a ratio of 22%:39%:39%. Even N- and S-glucosylations were achieved, a likewise unusual accomplishment.

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Language(s): eng - English
 Dates: 2021-01-182021-01-232021-02-03
 Publication Status: Published online
 Pages: 2
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.gresc.2021.01.007
 Degree: -

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Title: Green Synthesis and Catalysis
  Abbreviation : Green Synth. Catal.
Source Genre: Journal
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Publ. Info: Beijing : Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.
Pages: - Volume / Issue: 2 (1) Sequence Number: - Start / End Page: 4 - 5 Identifier: ISSN: 2666-5549
CoNE: https://pure.mpg.de/cone/journals/resource/2666-5549