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  The ESCRT-III machinery participates in the production of extracellular vesicles and protein export during Plasmodium falciparum infection

Avalos Padilla, Y., Georgiev, V., Lantero, E., Pujals, S., Verhoef, R., N. Borgheti-Cardoso, L., et al. (2021). The ESCRT-III machinery participates in the production of extracellular vesicles and protein export during Plasmodium falciparum infection. PLoS Pathogens, 17(4): e1009455. doi:10.1371/journal.ppat.1009455.

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Avalos Padilla, Yunuen1, Author              
Georgiev, Vasil1, Author              
Lantero, Elena, Author
Pujals, Silvia, Author
Verhoef, René, Author
N. Borgheti-Cardoso, Livia, Author
Albertazzi, Lorenzo, Author
Dimova, Rumiana1, Author              
Fernàndez-Busquets, Xavier, Author
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              

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Free keywords: Plasmodium; Membrane proteins; Cell membranes; Vesicles; Cytoplasm; Red blood cells; Parasitic diseases; Plasmodium falciparum
 Abstract: Malaria is a disease caused by Plasmodium parasites that is still a leading cause of death in many low-income countries, and for which currently available therapeutic strategies are not succeeding in its control, let alone eradication. An interesting feature observed after Plasmodium invasion is the increase of extracellular vesicles (EVs) generated by parasitized red blood cells (pRBCs), which lack a vesicular trafficking that would explain EV production. Here, by combining different approaches, we demonstrated the participation of the endosomal sorting complex required for transport (ESCRT) machinery from Plasmodium falciparum in the production of EVs in pRBCs. Moreover, we were able to detect ESCRT-III proteins adjacent to the membrane of the host and in EVs purified from a pRBC culture, which shows the export of these proteins and their participation in EV production. Finally, the disruption of an ESCRT-III associated gene, Pfvps60, led to a significant reduction in the amount of EVs. Altogether, these results confirm ESCRT-III participation in EV production and provide novel information on the P. falciparum protein export mechanisms, which can be used for the development of new therapeutic strategies against malaria, based on the disruption of EV formation and trafficking.

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Language(s): eng - English
 Dates: 2021-04-022021
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1371/journal.ppat.1009455
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Title: PLoS Pathogens
  Other : PLoS Pathog.
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 17 (4) Sequence Number: e1009455 Start / End Page: - Identifier: ISSN: 1553-7366