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  pH dependence of phosphate transport across the red blood cell membrane after modification by dansyl chloride

Berghout, A. G., Raida, M., Romano, L., & Passow, H. (1985). pH dependence of phosphate transport across the red blood cell membrane after modification by dansyl chloride. Biochimica et Biophysica Acta-Biomembranes, 815(2), 281-286. doi:10.1016/0005-2736(85)90298-6.

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 Creators:
Berghout, Alexander G.R.V.1, Author           
Raida, Manfred1, Author           
Romano, Leonardo1, Author           
Passow, Hermann1, Author           
Affiliations:
1Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              

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Free keywords: Anion transport; Phosphate transport; Dansyl chloride; Erythrocyte membrane; pH dependence
 Abstract: Dansylation of the red blood cell membrane inhibits monovalent anion transport as measured by means of 36Cl and enhances divalent anion transport as measured by means of 35SO4 (Legrum, Fasold and Passow (1980) Hoppe-Seyler's Z. Physiol. Chem. 361, 1573–1590 and Lepke and Passow (1982) J. Physiol. (London) 328, 27–48). In the present work the effect of densylation on phosphate equilibrium exchange was studied over the pH range where the ratio between monovalent and divalent phosphate anions varies. At high pH, phosphate equilibrium exchange was enhanced; at low pH, exchange was inhibited. The pH maximum of phosphate equilibrium exchange, seen at pH 6.3 in untreated ghosts is now replaced by a plateau. The inverse effects of dansylation on the rates of exchange at high and low pH suggest that both monovalent and divalent phosphate anions are accepted as substrates by the anion transport protein. A tentative attempt to obtain a quantitative estimate of the ratio of monovalent and divalent phosphate transport indicates that in the untreated red cell membrane over the pH range 7.2–8.5 the transport of HPO42− is negligible compared to the transport of H2PO4.

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Language(s): eng - English
 Dates: 1984-10-221985-05-18
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0005-2736(85)90298-6
PMID: 3995030
 Degree: -

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Title: Biochimica et Biophysica Acta-Biomembranes
Source Genre: Journal
 Creator(s):
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 815 (2) Sequence Number: - Start / End Page: 281 - 286 Identifier: ISSN: 0005-2736
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702