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  Automated suppression of sample-related artifacts in Fluorescence Correlation Spectroscopy

Ries, J., Bayer, M., Csucs, G., Dirkx, R., Solimena, M., Ewers, H., et al. (2010). Automated suppression of sample-related artifacts in Fluorescence Correlation Spectroscopy. Optics Express, 18(11), 11073-11082. doi:10.1364/OE.18.011073.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-4B74-2 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-4B75-1
Genre: Journal Article

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oe-18-11-11073.pdf (Publisher version), 766KB
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 Creators:
Ries, Jonas1, Author
Bayer, Mathias1, Author
Csucs, Gabor1, Author
Dirkx, Ronald1, Author
Solimena, Michele1, Author
Ewers, Helge1, Author
Schwille, Petra1, Author
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1external, ou_persistent22              

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Free keywords: CROSS-CORRELATION SPECTROSCOPY; LIVING CELLSOptics;
 Abstract: Fluorescence Correlation Spectroscopy (FCS) in cells often suffers from artifacts caused by bright aggregates or vesicles, depletion of fluorophores or bleaching of a fluorescent background. The common practice of manually discarding distorted curves is time consuming and subjective. Here we demonstrate the feasibility of automated FCS data analysis with efficient rejection of corrupted parts of the signal. As test systems we use a solution of fluorescent molecules, contaminated with bright fluorescent beads, as well as cells expressing a fluorescent protein (ICA512-EGFP), which partitions into bright secretory granules. This approach improves the accuracy of FCS measurements in biological samples, extends its applicability to especially challenging systems and greatly simplifies and accelerates the data analysis. (C) 2010 Optical Society of America

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Language(s): eng - English
 Dates: 2010
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000278512300018
DOI: 10.1364/OE.18.011073
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Grant ID : SO 818/1-1
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Funding organization : DFG

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Title: Optics Express
  Abbreviation : Opt. Express
Source Genre: Journal
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Publ. Info: Washington, DC : Optical Society of America
Pages: - Volume / Issue: 18 (11) Sequence Number: - Start / End Page: 11073 - 11082 Identifier: ISSN: 1094-4087
CoNE: https://pure.mpg.de/cone/journals/resource/954925609918