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Rat proximal tubule; Intracellular Na+ concentration; Na+/H+ counter transport; Na+(HCO−3)2 cotransport
Abstract:
The intracellular Na+ concentration of surface loops of proximal tubules was measured with doublebarrelled Na+-sensitive microelectrodes in micropuncture experiments on rat kidneys in situ and in vivo. With the help of a fast recording system, it was possible to select only valid measurements, by discarding all records in which the Na+ concentration rose after the impalement presumably as a result of sodium ion inflow via puncture leaks. Under free-flow conditions cell Na+ activity averaged 13.1, S.D.±2.1 mmol/l which, assuming an activity coefficient of 0.75, corresponds to a Na+ concentration of 17.5 mmol/l. The simultaneously recorded cell membrane potential was −73.5 mV in agreement with best estimates derived previously. The comparison of cell sodium activity and cell pH (Yoshitomi and Frömter [17]) demonstrates that the electrochemical potential difference of Na+ is greater than that of H+, as required for the operation of Na+/H+ counter transport in the brushborder membrane but that bicarbonate efflux via the sodium bicarbonate contransport system at the peritubular cell membrane requires a HCO−3 to Na+ stoichiometry of greater than 2.0 or the involvement of other ions at least under free-flow conditions.
