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  Longitudinal development of antibody responses in COVID-19 patients of different severity with ELISA, peptide, and glycan arrays : an immunological case series

Heidepriem, J., Dahlke, C., Kobbe, R., Santer, R., Koch, T., Fathi, A., et al. (2021). Longitudinal development of antibody responses in COVID-19 patients of different severity with ELISA, peptide, and glycan arrays: an immunological case series. Pathogens, 10(4): 438. doi:10.3390/pathogens10040438.

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 Creators:
Heidepriem, Jasmin1, Author              
Dahlke, Christine, Author
Kobbe, Robin, Author
Santer, René, Author
Koch, Till, Author
Fathi, Anahita, Author
Silva Seco, Bruna Mara2, Author              
Ly, My L., Author
Schmiedel, Stefan, Author
Schwinge, Dorothee, Author
Serna, Sonia, Author
Sellrie, Katrin3, Author              
Reichardt, Niels-Christian, Author
Seeberger, Peter H.2, Author              
Addo, Marylyn M., Author
Löffler, Felix F.1, Author              
ID-UKE COVID-19 Study, Author
Affiliations:
1Felix Löffler, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2385692              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              
3Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863286              

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Free keywords: SARS-CoV-2; COVID-19; full proteome; peptide microarrays; glycan microarrays
 Abstract: The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). A better understanding of its immunogenicity can be important for the development of improved diagnostics, therapeutics, and vaccines. Here, we report the longitudinal analysis of three COVID-19 patients with moderate (1) and mild disease (2 and 3). Antibody serum responses were analyzed using spike glycoprotein enzyme linked immunosorbent assay (ELISA), full-proteome peptide, and glycan microarrays. ELISA immunoglobulin A, G, and M (IgA, IgG, and IgM) signals increased over time for individuals 1 and 2, whereas 3 only showed no clear positive IgG and IgM result. In contrast, peptide microarrays showed increasing IgA/G signal intensity and epitope spread only in the moderate patient 1 over time, whereas early but transient IgA and stable IgG responses were observed in the two mild cases 2 and 3. Glycan arrays showed an interaction of antibodies to fragments of high-mannose and core N-glycans, present on the viral shield. In contrast to protein ELISA, microarrays allow for a deeper understanding of IgA, IgG, and IgM antibody responses to specific epitopes of the whole proteome and glycans of SARS-CoV-2 in parallel. In the future, this may help to better understand and to monitor vaccination programs and monoclonal antibodies as therapeutics.

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Language(s): eng - English
 Dates: 2021-04-062021
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.3390/pathogens10040438
DOI: 10.1101/2020.04.14.20059733
BibTex Citekey: pathogens10040438
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Title: Pathogens
Source Genre: Journal
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Publ. Info: Basel : MDPI
Pages: - Volume / Issue: 10 (4) Sequence Number: 438 Start / End Page: - Identifier: ISSN: 2076-0817

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Title: medRxiv
Source Genre: Journal
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Publ. Info: Cold Spring Harbor : Cold Spring Harbor Laboratory
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