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  Combined AFM and two-focus SFCS study of raft-exhibiting model membranes

Chiantia, S., Ries, J., Kahya, N., & Schwille, P. (2006). Combined AFM and two-focus SFCS study of raft-exhibiting model membranes. ChemPhysChem, 7(11), 2409-2418. doi:10.1002/cphc.200600464.

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Chiantia, Salvatore1, Author
Ries, Jonas1, Author
Kahya, Nicoletta1, Author
Schwille, Petra2, Author              
Affiliations:
1external, ou_persistent22              
2Biophysics/BIOTEC, TU Dresden, ou_persistent22              

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Free keywords: PLACENTAL ALKALINE-PHOSPHATASE; ATOMIC-FORCE MICROSCOPY; GPI-ANCHORED PROTEINS; FLUORESCENCE CORRELATION; LIPID-BILAYERS; SUPPORTED BILAYERS; DOMAIN-STRUCTURE; NANOMECHANICS; MICRODOMAINS; DETERMINESChemistry; Physics; fluorercent probes; force measurements; membranes; spingolipids;
 Abstract: Dioleoylphosphatidylcholine/sphingomyelin/cholesterol (DOPC/SM/cholesterol) model membranes exhibit liquid-liquid phase separation and therefore provide a physical model for the putative liquid-ordered domains present in cells. Here we present a combination of atomic force microscopy (AFM) imaging, force measurements, confocal fluorescence imaging and two-focus scanning fluorescence correlation spectroscopy (two-focus SFCS) to obtain structural and dynamical information about this model membrane system. Partition coefficients and diffusion coefficiengs in the different phases were measured with two-focus SFCS for numerous fluorescent lipid analogues and proteins, while being directly related to the lateral organization of the membrane and its mechanical properties probed by AFM. Moreover we show how the combination of these different approaches is effective in reducing artifacts resulting from the use of a single technique.

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Language(s): eng - English
 Dates: 2006
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000242319200023
DOI: 10.1002/cphc.200600464
 Degree: -

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Title: ChemPhysChem
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 7 (11) Sequence Number: - Start / End Page: 2409 - 2418 Identifier: ISSN: 1439-4235
CoNE: https://pure.mpg.de/cone/journals/resource/954925409790