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  Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging

Lange, F., Agüi-Gonzalez, P., Riedel, D., Phan, N. T. N., Jakobs, S., & Rizzoli, S. O. (2020). Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging. bioRxiv, 10.1101/2020.10.05.326074. doi:10.1101/2020.10.05.326074.

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Lange, F.1, Autor           
Agüi-Gonzalez, P., Autor
Riedel, D.2, Autor           
Phan, N. T. N., Autor
Jakobs, S.1, Autor           
Rizzoli, S. O., Autor
Affiliations:
1Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Zusammenfassung: 1
Title:
Correlative fluorescence microscopy, transmission electron microscopy and
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secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging
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Felix Lange
1,2,

, Paola Agüi-Gonzalez
3,4,

, Dietmar Riedel
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, Nhu T.N. Phan
3,4
, Stefan Jakobs
1,2,4,&
,
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Silvio O. Rizzoli
3,4,&
5
1
Research Group Mitochondrial Structure and Dynamics, Max Planck Institute for Biophysical
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Chemistry, Göttingen, Germany
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2
Clinic for Neurology, University Medical Center Göttingen, Germany
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3
Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Germany
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4
Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen,
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Germany
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5
Laboratory of Electron Microscopy, Max Planck Institute for Biophysical Chemistry, Göttingen,
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Germany
13

These authors contributed equally to this work.
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&
These authors also contributed equally to this work.
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*
Correspondence to: sjakobs@gwdg.de; srizzol@gwdg.de
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Abstract
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Electron microscopy (EM) has been employed for decades to analyze cell structure. To also
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analyze the positions and functions of specific proteins, one typically relies on immuno-EM or on
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a correlation with fluorescence microscopy, in the form of correlated light and electron microscopy
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(CLEM). Nevertheless, neither of these procedures is able to also address the isotopic
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composition of cells. To solve this, a correlation with secondary ion mass spectrometry (SIMS)
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would be necessary. SIMS has been correlated in the past to EM or to fluorescence microscopy
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in biological samples, but not to CLEM. We achieved this here, using a protocol based on
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transmission EM, conventional epifluorescence microscopy and nanoSIMS. The protocol is easily
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applied, and enables the use of all three technologies at high performance parameters. We
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suggest that CLEM-SIMS will provide substantial information that is currently beyond the scope of

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Sprache(n): eng - English
 Datum: 2020-10-05
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Keine Begutachtung
 Identifikatoren: DOI: 10.1101/2020.10.05.326074
 Art des Abschluß: -

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Titel: bioRxiv
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: - Artikelnummer: 10.1101/2020.10.05.326074 Start- / Endseite: - Identifikator: -