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  Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging

Lange, F., Agüi-Gonzalez, P., Riedel, D., Phan, N. T. N., Jakobs, S., & Rizzoli, S. O. (2020). Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging. bioRxiv, 10.1101/2020.10.05.326074. doi:10.1101/2020.10.05.326074.

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Lange, F.1, Author              
Agüi-Gonzalez, P., Author
Riedel, D.2, Author              
Phan, N. T. N., Author
Jakobs, S.1, Author              
Rizzoli, S. O., Author
Affiliations:
1Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Abstract: 1 Title: Correlative fluorescence microscopy, transmission electron microscopy and 2 secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging 3 Felix Lange 1,2, ¶ , Paola Agüi-Gonzalez 3,4, ¶ , Dietmar Riedel 5 , Nhu T.N. Phan 3,4 , Stefan Jakobs 1,2,4,& , 4 Silvio O. Rizzoli 3,4,& 5 1 Research Group Mitochondrial Structure and Dynamics, Max Planck Institute for Biophysical 6 Chemistry, Göttingen, Germany 7 2 Clinic for Neurology, University Medical Center Göttingen, Germany 8 3 Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Germany 9 4 Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, 10 Germany 11 5 Laboratory of Electron Microscopy, Max Planck Institute for Biophysical Chemistry, Göttingen, 12 Germany 13 ¶ These authors contributed equally to this work. 14 & These authors also contributed equally to this work. 15 * Correspondence to: sjakobs@gwdg.de; srizzol@gwdg.de 16 17 18 Abstract 19 Electron microscopy (EM) has been employed for decades to analyze cell structure. To also 20 analyze the positions and functions of specific proteins, one typically relies on immuno-EM or on 21 a correlation with fluorescence microscopy, in the form of correlated light and electron microscopy 22 (CLEM). Nevertheless, neither of these procedures is able to also address the isotopic 23 composition of cells. To solve this, a correlation with secondary ion mass spectrometry (SIMS) 24 would be necessary. SIMS has been correlated in the past to EM or to fluorescence microscopy 25 in biological samples, but not to CLEM. We achieved this here, using a protocol based on 26 transmission EM, conventional epifluorescence microscopy and nanoSIMS. The protocol is easily 27 applied, and enables the use of all three technologies at high performance parameters. We 28 suggest that CLEM-SIMS will provide substantial information that is currently beyond the scope of

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Language(s): eng - English
 Dates: 2020-10-05
 Publication Status: Published online
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 Rev. Type: No review
 Identifiers: DOI: 10.1101/2020.10.05.326074
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Title: bioRxiv
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: 10.1101/2020.10.05.326074 Start / End Page: - Identifier: -