English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Stress-induced nuclear condensation of NELF drives transcriptional downregulation

Rawat, P., Böhning, M., Hummel, B., Aprile-Garcia, F., Pandit, A. S., Eisenhardt, N., et al. (2021). Stress-induced nuclear condensation of NELF drives transcriptional downregulation. Molecular Cell, 81(5), 1013-1026.e11. doi:10.1016/j.molcel.2021.01.016.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
3310720.pdf (Publisher version), 5MB
Name:
3310720.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Rawat, P., Author
Böhning, M.1, Author           
Hummel, B., Author
Aprile-Garcia, F., Author
Pandit, A. S., Author
Eisenhardt, N., Author
Khavaran, A., Author
Niskanen, E., Author
Vos, S. M., Author
Palvimo, J. J., Author
Pichler, A., Author
Cramer, P.1, Author           
Sawarkar, R., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: negative elongation factor (NELF); RNA polymerase II; heat shock; proteostasis; transcriptional stress response; pausing; CDK9; phase separation; transcriptional condensates; SUMO
 Abstract: In response to stress, human cells coordinately downregulate transcription and translation of housekeeping genes. To downregulate transcription, the negative elongation factor (NELF) is recruited to gene promoters impairing RNA polymerase II elongation. Here we report that NELF rapidly forms nuclear condensates upon stress in human cells. Condensate formation requires NELF dephosphorylation and SUMOylation induced by stress. The intrinsically disordered region (IDR) in NELFA is necessary for nuclear NELF condensation and can be functionally replaced by the IDR of FUS or EWSR1 protein. We find that biomolecular condensation facilitates enhanced recruitment of NELF to promoters upon stress to drive transcriptional downregulation. Importantly, NELF condensation is required for cellular viability under stressful conditions. We propose that stress-induced NELF condensates reported here are nuclear counterparts of cytosolic stress granules. These two stress-inducible condensates may drive the coordinated downregulation of transcription and translation, likely forming a critical node of the stress survival strategy.

Details

show
hide
Language(s): eng - English
 Dates: 2021-02-052021-03-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2021.01.016
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 81 (5) Sequence Number: - Start / End Page: 1013 - 1026.e11 Identifier: -