English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Transcription activation depends on the length of the RNA polymerase II C‐terminal domain

Sawicka, A., Villamil, G., Lidschreiber, M., Darzacq, X., Dugast‐Darzacq, C., Schwalb, B., et al. (2021). Transcription activation depends on the length of the RNA polymerase II C‐terminal domain. EMBO Journal, 40(9): e107015. doi:10.15252/embj.2020107015.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
3310736.pdf (Publisher version), 5MB
Name:
3310736.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Sawicka, A.1, Author              
Villamil, G.1, Author              
Lidschreiber, M.1, Author              
Darzacq, X., Author
Dugast‐Darzacq, C., Author
Schwalb, B.1, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: RNA polymerase II; enhancer; gene expression; transcription kinetics
 Abstract: Eukaryotic RNA polymerase II (Pol II) contains a tail‐like, intrinsically disordered carboxy‐terminal domain (CTD) comprised of heptad‐repeats, that functions in coordination of the transcription cycle and in coupling transcription to co‐transcriptional processes. The CTD repeat number varies between species and generally increases with genome size, but the reasons for this are unclear. Here, we show that shortening the CTD in human cells to half of its length does not generally change pre‐mRNA synthesis or processing in cells. However, CTD shortening decreases the duration of promoter‐proximal Pol II pausing, alters transcription of putative enhancer elements, and delays transcription activation after stimulation of the MAP kinase pathway. We suggest that a long CTD is required for efficient enhancer‐dependent recruitment of Pol II to target genes for their rapid activation.

Details

show
hide
Language(s): eng - English
 Dates: 2021-02-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.15252/embj.2020107015
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: EMBO Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 17 Volume / Issue: 40 (9) Sequence Number: e107015 Start / End Page: - Identifier: -