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  Transcription activation depends on the length of the RNA polymerase II C‐terminal domain

Sawicka, A., Villamil, G., Lidschreiber, M., Darzacq, X., Dugast‐Darzacq, C., Schwalb, B., et al. (2021). Transcription activation depends on the length of the RNA polymerase II C‐terminal domain. EMBO Journal, 40(9): e107015. doi:10.15252/embj.2020107015.

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 Creators:
Sawicka, A.1, Author           
Villamil, G.1, Author           
Lidschreiber, M.1, Author           
Darzacq, X., Author
Dugast‐Darzacq, C., Author
Schwalb, B.1, Author           
Cramer, P.1, Author           
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: RNA polymerase II; enhancer; gene expression; transcription kinetics
 Abstract: Eukaryotic RNA polymerase II (Pol II) contains a tail‐like, intrinsically disordered carboxy‐terminal domain (CTD) comprised of heptad‐repeats, that functions in coordination of the transcription cycle and in coupling transcription to co‐transcriptional processes. The CTD repeat number varies between species and generally increases with genome size, but the reasons for this are unclear. Here, we show that shortening the CTD in human cells to half of its length does not generally change pre‐mRNA synthesis or processing in cells. However, CTD shortening decreases the duration of promoter‐proximal Pol II pausing, alters transcription of putative enhancer elements, and delays transcription activation after stimulation of the MAP kinase pathway. We suggest that a long CTD is required for efficient enhancer‐dependent recruitment of Pol II to target genes for their rapid activation.

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Language(s): eng - English
 Dates: 2021-02-08
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.15252/embj.2020107015
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Title: EMBO Journal
Source Genre: Journal
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Pages: 17 Volume / Issue: 40 (9) Sequence Number: e107015 Start / End Page: - Identifier: -