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  Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 Å in cells

Tegunov, D., Xue, L., Dienemann, C., Cramer, P., & Mahamid, J. (2021). Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 Å in cells. Nature Methods, 18(2), 186-193. doi:10.1038/s41592-020-01054-7.

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 Creators:
Tegunov, D.1, Author              
Xue, L., Author
Dienemann, C.1, Author              
Cramer, P.1, Author              
Mahamid, J., Author
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: Electron microscopy; Software; Structure determination
 Abstract: Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro and inside cells. In addition to aligning individual particles, accurate registration of sample motion and three-dimensional deformation during exposures are crucial for achieving high-resolution reconstructions. Here we describe M, a software tool that establishes a reference-based, multi-particle refinement framework for cryo-EM data and couples a comprehensive spatial deformation model to in silico correction of electron-optical aberrations. M provides a unified optimization framework for both frame-series and tomographic tilt-series data. We show that tilt-series data can provide the same resolution as frame-series data on a purified protein specimen, indicating that the alignment step no longer limits the resolution obtainable from tomographic data. In combination with Warp and RELION, M resolves to residue level a 70S ribosome bound to an antibiotic inside intact bacterial cells. Our work provides a computational tool that facilitates structural biology in cells.

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Language(s): eng - English
 Dates: 2021-02-042021-02
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-020-01054-7
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Title: Nature Methods
Source Genre: Journal
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Pages: - Volume / Issue: 18 (2) Sequence Number: - Start / End Page: 186 - 193 Identifier: -