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  Membrane-coated 3D architectures for bottom-up synthetic biology dagger

Eto, H., Franquelim, H. G., Heymann, M., & Schwille, P. (2021). Membrane-coated 3D architectures for bottom-up synthetic biology dagger. Soft Matter, 17, 5456-5466. doi:10.1039/d1sm00112d.

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d1sm00112d.pdf (Publisher version), 5MB
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 Creators:
Eto, Hiromune1, Author              
Franquelim, Henri G.1, Author              
Heymann, Michael1, Author              
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: Chemistry; Materials Science; Physics; Polymer Science;
 Abstract: One of the great challenges of bottom-up synthetic biology is to recreate the cellular geometry and surface functionality required for biological reactions. Of particular interest are lipid membrane interfaces where many protein functions take place. However, cellular 3D geometries are often complex, and custom-shaping stable lipid membranes on relevant spatial scales in the micrometer range has been hard to accomplish reproducibly. Here, we use two-photon direct laser writing to 3D print microenvironments with length scales relevant to cellular processes and reactions. We formed lipid bilayers on the surfaces of these printed structures, and we evaluated multiple combinatorial scenarios, where physiologically relevant membrane compositions were generated on several different polymer surfaces. Functional dynamic protein systems were reconstituted in vitro and their self-organization was observed in response to the 3D geometry. This method proves very useful to template biological membranes with an additional spatial dimension, and thus allows a better understanding of protein function in relation to the complex morphology of cells and organelles.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: We also thank the Biochemistry Core Facility of the Max Planck Institute of Biochemistry for assistance with protein purification, and the Imaging Core Facility of the same institution for assistance on the 4D image visualisation.
 Rev. Type: Peer
 Identifiers: ISI: 000634847200001
DOI: 10.1039/d1sm00112d
 Degree: -

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Title: Soft Matter
  Abbreviation : Soft Matter
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 17 Sequence Number: - Start / End Page: 5456 - 5466 Identifier: ISSN: 1744-683X
CoNE: https://pure.mpg.de/cone/journals/resource/1744-683X