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  Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection

Martin-Jaular, L., Nevo, N., Schessner, J. P., Tkach, M., Jouve, M., Dingli, F., et al. (2021). Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO Journal, 40: e105492. doi:10.15252/embj.2020105492.

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 Creators:
Martin-Jaular, Lorena1, Author
Nevo, Nathalie1, Author
Schessner, Julia P.2, Author              
Tkach, Mercedes1, Author
Jouve, Mabel1, Author
Dingli, Florent1, Author
Loew, Damarys1, Author
Witwer, Kenneth W.1, Author
Ostrowski, Matias1, Author
Borner, Georg H. H.3, Author              
Thery, Clotilde1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
3Borner, Georg / Systems Biology of Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_3060205              

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Free keywords: T-CELLS; PROTEIN; NEF; EXOSOMES; REVEALS; CD45; IDENTIFICATION; MICROVESICLES; PURIFICATION; ACTIVATIONBiochemistry & Molecular Biology; Cell Biology; exosomes; extracellular vesicles; HIV; proteomics; T cells;
 Abstract: Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surrounding cells. Specific markers to distinguish different EVs (e.g. exosomes, ectosomes, enveloped viruses like HIV) are still lacking. We have developed a proteomic profiling approach for characterizing EV subtype composition and applied it to human Jurkat T cells. We generated an interactive database to define groups of proteins with similar profiles, suggesting release in similar EVs. Biochemical validation confirmed the presence of preferred partners of commonly used exosome markers in EVs: CD81/ADAM10/ITGB1, and CD63/syntenin. We then compared EVs from control and HIV-1-infected cells. HIV infection altered EV profiles of several cellular proteins, including MOV10 and SPN, which became incorporated into HIV virions, and SERINC3, which was re-routed to non-viral EVs in a Nef-dependent manner. Furthermore, we found that SERINC3 controls the surface composition of EVs. Our workflow provides an unbiased approach for identifying candidate markers and potential regulators of EV subtypes. It can be widely applied to in vitro experimental systems for investigating physiological or pathological modifications of EV release.

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Language(s): eng - English
 Dates: 2021-03
 Publication Status: Published online
 Pages: 25
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000627711900001
DOI: 10.15252/embj.2020105492
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Project name : Gottfried Wilhelm Leibniz Prize MA 1764/2-1
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Funding organization : German Research Foundation

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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 40 Sequence Number: e105492 Start / End Page: - Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061