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  Structural basis of nucleosome transcription mediated by Chd1 and FACT

Farnung, L., Ochmann, M., Engeholm, M., & Cramer, P. (2021). Structural basis of nucleosome transcription mediated by Chd1 and FACT. Nature Structural and Molecular Biology, 28(4), 382-387. doi:10.1038/s41594-021-00578-6.

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 Creators:
Farnung, L.1, Author              
Ochmann, M.1, Author              
Engeholm, M.1, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: Chromatin remodelling; DNA; Electron microscopy; Nucleosomes; Transcription
 Abstract: Efficient transcription of RNA polymerase II (Pol II) through nucleosomes requires the help of various factors. Here we show biochemically that Pol II transcription through a nucleosome is facilitated by the chromatin remodeler Chd1 and the histone chaperone FACT when the elongation factors Spt4/5 and TFIIS are present. We report cryo-EM structures of transcribing Saccharomyces cerevisiae Pol II−Spt4/5−nucleosome complexes with bound Chd1 or FACT. In the first structure, Pol II transcription exposes the proximal histone H2A−H2B dimer that is bound by Spt5. Pol II has also released the inhibitory DNA-binding region of Chd1 that is poised to pump DNA toward Pol II. In the second structure, Pol II has generated a partially unraveled nucleosome that binds FACT, which excludes Chd1 and Spt5. These results suggest that Pol II progression through a nucleosome activates Chd1, enables FACT binding and eventually triggers transfer of FACT together with histones to upstream DNA.

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Language(s): eng - English
 Dates: 2021-04-122021-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41594-021-00578-6
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 28 (4) Sequence Number: - Start / End Page: 382 - 387 Identifier: -