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  Implantable photonic neural probes for light-sheet fluorescence brain imaging

Sacher, W. D., Chen, F.-D., Moradi-Chameh, H., Luo, X., Fomenko, A., Shah, P., et al. (2021). Implantable photonic neural probes for light-sheet fluorescence brain imaging. Neurophotonics, 8(2): 025003. doi:10.1117/1.NPh.8.2.025003.

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https://doi.org/10.1117/1.NPh.8.2.025003 (Publisher version)
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Sacher, Wesley D.1, Author              
Chen, Fu-Der2, Author
Moradi-Chameh, Homeira2, Author
Luo, Xianshu2, Author
Fomenko, Anton2, Author
Shah, Prajay2, Author
Lordello, Thomas2, Author
Liu, Xinyu2, Author
Almog, Ilan Felts2, Author
Straguzzi, John N.1, Author              
Fowler, Trevor M.2, Author
Jung, Youngho1, Author              
Hu, Ting2, Author
Jeong, Junho2, Author
Lozano, Andres M.2, Author
Lo, Patrick Guo-Qiang2, Author
Valiante, Taufik A.2, Author
Moreaux, Laurent C.2, Author
Poon, Joyce K. S.1, Author              
Roukes, Michael L.2, Author
Affiliations:
1Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society, ou_3287471              
2External Organizations, ou_persistent22              

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 Abstract: Significance: Light-sheet fluorescence microscopy (LSFM) is a powerful technique for highspeed volumetric functional imaging. However, in typical light-sheet microscopes, the illumination and collection optics impose significant constraints upon the imaging of non-transparent brain tissues. We demonstrate that these constraints can be surmounted using a new class of implantable photonic neural probes. Aim: Mass manufacturable, silicon-based light-sheet photonic neural probes can generate planar patterned illumination at arbitrary depths in brain tissues without any additional micro-optic components. Approach: We develop implantable photonic neural probes that generate light sheets in tissue. The probes were fabricated in a photonics foundry on 200-mm-diameter silicon wafers. The light sheets were characterized in fluorescein and in free space. The probe-enabled imaging approach was tested in fixed, in vitro, and in vivo mouse brain tissues. Imaging tests were also performed using fluorescent beads suspended in agarose. Results: The probes had 5 to 10 addressable sheets and average sheet thicknesses <16 μm for propagation distances up to 300 μm in free space. Imaging areas were as large as ≈240 μm × 490 μm in brain tissue. Image contrast was enhanced relative to epifluorescence microscopy. . Conclusions: The neural probes can lead to new variants of LSFM for deep brain imaging and experiments in freely moving animals.

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 Dates: 2021-04-19
 Publication Status: Published online
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 Identifiers: DOI: 10.1117/1.NPh.8.2.025003
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Title: Neurophotonics
Source Genre: Journal
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Publ. Info: Bellingham, WA, USA : SPIE
Pages: - Volume / Issue: 8 (2) Sequence Number: 025003 Start / End Page: - Identifier: ISSN: 2329-4248
CoNE: https://pure.mpg.de/cone/journals/resource/2329-4248