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  Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons

Perez, J. D., Tom Dieck, S., Alvarez-Castelao, B., Tushev, G., Chan, I. C., & Schuman, E. M. (2021). Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons. eLife, 10: 63092. doi:10.7554/eLife.63092.

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Genre: Journal Article

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https://elifesciences.org/articles/63092 (Publisher version)
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 Creators:
Perez, J. D.1, Author
Tom Dieck, S.1, Author
Alvarez-Castelao, B.1, Author
Tushev, G.1, Author
Chan, I. C.1, 2, Author
Schuman, Erin Margaret3, Author              
Affiliations:
1Max Planck Institute for Brain Research, Max Planck Society, Max-von-Laue-Str. 4, 60438 Frankfurt am Main, DE, ou_2461692              
2Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society, Ludwig-Erhard-Allee 2, 53175 Bonn, DE, ou_2173678              
3Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              

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Free keywords: GABAergic; local translation; neuroscience; rat; transcriptome
 Abstract: Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell-types is unknown. Here we describe the unbiased transcriptomic characterization of the subcellular compartments of hundreds of single neurons. We separately profiled the dendritic and somatic transcriptomes of individual rat hippocampal neurons and investigated mRNA abundances in the soma and dendrites of single glutamatergic and GABAergic neurons. We found that, like their excitatory counterparts, interneurons contain a rich repertoire of ~4000 mRNAs. We observed more cell type-specific features among somatic transcriptomes than their associated dendritic transcriptomes. Finally, using cell-type specific metabolic labelling of isolated neurites, we demonstrated that the processes of Glutamatergic and, notably, GABAergic neurons were capable of local translation, suggesting mRNA localization and local translation is a general property of neurons.

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Language(s): eng - English
 Dates: 2021-01-07
 Publication Status: Published in print
 Pages: 26
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: PMID: 33404500
DOI: 10.7554/eLife.63092
 Degree: -

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Title: eLife
  Abbreviation : Elife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 10 Sequence Number: 63092 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X