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  Lint, a transmembrane serine protease, regulates growth and metabolism in Drosophila

Pathak, H., Maya, A. V., Tanari, A. B., Sarkar, S., & Varghese, J. (2021). Lint, a transmembrane serine protease, regulates growth and metabolism in Drosophila. Genetics. doi:10.1093/genetics/iyab035.

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Pathak et al..pdf (Publisher version), 782KB
 
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 Creators:
Pathak, Himani1, Author
Maya, Ananthakrishnan Vijaykumar2, Author
Tanari, Abdul Basith1, Author
Sarkar, Sohela1, Author
Varghese, Jishy1, Author
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1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              

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Free keywords: growth; hypoxia; insulin signalling; trachea
 Abstract: nsulin signaling in Drosophila has a significant role in regulating growth, metabolism, fecundity, stress response, and longevity. The molecular mechanism by which insulin signaling regulates these vital processes is dependent on the nutrient status and oxygen availability of the organism. In a genetic screen to identify novel genes that regulate Drosophila insulin signalling, we discovered Lumens Interrupted (lint), a gene that has previously been shown to act in tracheal development. The knockdown of lint gene expression using a Dilp2Gal4 driver which expresses in the neuronal insulin-producing cells (IPCs), led to defects in systemic insulin signaling, metabolic status and growth. However, our analysis of lint knockdown phenotypes revealed that downregulation of lint in the trachea and not IPCs was responsible for the growth phenotypes, as the Gal4 driver is also expressed in the tracheal system. We found various tracheal terminal branch defects, including reduction in the length as well as number of branches in the lint knockdown background. Our study reveals that substantial effects of lint downregulation arose because of tracheal defects, which induced tissue hypoxia, altered systemic insulin/TOR signaling, and resulted in effects on developmental growth regulation.

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Language(s): eng - English
 Dates: 2021-03-03
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1093/genetics/iyab035
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Title: Genetics
Source Genre: Journal
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Publ. Info: Genetics Society of America
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0016-6731
CoNE: https://pure.mpg.de/cone/journals/resource/954925400554