English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Next generation cell culture tools featuring micro‐ and nanotopographies for biological screening

Carthew, J., Abdelmaksoud, H. H., Cowley, K. J., Hodgson‐Garms, M., Elnathan, R., Spatz, J. P., et al. (2022). Next generation cell culture tools featuring micro‐ and nanotopographies for biological screening. Advanced Functional Materials, 32(3): 2100881, pp. 1-15. doi:10.1002/adfm.202100881.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
AdvFunctMater_32_2021_2100881.pdf (Any fulltext), 7MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2021_2100881.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2021_2100881_Suppl.pdf (Supplementary material), 8MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2021_2100881_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Carthew, James, Author
Abdelmaksoud, Hazem H., Author
Cowley, Karla J., Author
Hodgson‐Garms, Margeaux, Author
Elnathan, Roey, Author
Spatz, Joachim P.1, Author              
Brugger, Juergen, Author
Thissen, Helmut, Author
Simpson, Kaylene J., Author
Voelcker, Nicolas H., Author
Frith, Jessica E., Author
Cadarso, Victor J., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: high-throughput screening, micro/nano-topography, nanoimprint lithography, tissue culture plastic
 Abstract: Cells can perceive complex mechanical cues across both the micro‐ and nanoscale which can influence their development. While causative effects between surface topography and cellular function can be demonstrated, the variability in materials used in this screening process makes it difficult to discern whether the observed phenotypic changes are indeed a result of topographical cues alone or the inherent difference in material properties. A novel approach to directly imprint micro‐ and nanoscaled topographical features into the base of conventional cell cultureware is thus developed, facilitating its compatibility with standard biological techniques and methods of analysis. The utility of this technology is demonstrated by performing high‐throughput screening across five distinct cell types to interrogate the effects of 12 surface topographies, exemplifying unique cell‐specific responses to both behavior and cell morphological characteristics. The ability of this technology to underpin new insights into how surface topographies can regulate key image descriptors to drive cell fate determination is further demonstrated. These findings will inform the future development of advanced micro‐ and nanostructured cell culture substrates that can regulate cell behavior and fate determination across the life sciences, including fundamental cell biology, drug screening, and cell therapy.

Details

show
hide
Language(s): eng - English
 Dates: 2021-03-122021-01-272021-04-212022-01-14
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/adfm.202100881
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Advanced Functional Materials
  Other : Adv. Funct. Mater.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: 32 (3) Sequence Number: 2100881 Start / End Page: 1 - 15 Identifier: ISSN: 1616-301X
CoNE: https://pure.mpg.de/cone/journals/resource/954925596563