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  Future feed control: tracing banned bovine material in insect meal

Belghit, I., Varunjikar, M., Lecrenier, M.-C., Steinhilber, A., Niedzwiecka, A., Wang, Y., et al. (2021). Future feed control: tracing banned bovine material in insect meal. Food control, 108183. doi:10.1016/j.foodcont.2021.108183.

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 Creators:
Belghit, I., Author
Varunjikar, M., Author
Lecrenier, M.-C., Author
Steinhilber, A.E., Author
Niedzwiecka, A., Author
Wang, Yiming1, Author              
Dieu, M., Author
Azzollini, D., Author
Lie, K., Author
Lock, E.-J., Author
Berntssen, M.H.G., Author
Renard, P., Author
Zagon, J., Author
Fumière, O., Author
van Loon, J.J.A., Author
Larsen, Thomas1, Author              
Poetz, O., Author
Braeuning, A., Author
Palmblad, M., Author
Rasinger, J.D., Author
Affiliations:
1Archaeology, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074312              

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Free keywords: Feed control, BSF larvae, Proteomics, Carbon isotope fingerprinting of amino acids, qPCR, Spectral libraries
 Abstract: In the present study, we assessed if different legacy and novel molecular analyses approaches can detect and trace prohibited bovine material in insects reared to produce processed animal protein (PAP). Newly hatched black soldier fly (BSF) larvae were fed one of the four diets for seven days; a control feeding medium (Ctl), control feed spiked with bovine hemoglobin powder (BvHb) at 1% (wet weight, w/w) (BvHb 1%, w/w), 5% (BvHb 5%, w/w) and 10% (BvHb 10%, w/w). Another dietary group of BSF larvae, namely *BvHb 10%, were first grown on BvHb 10% (w/w), and after seven days separated from the residual material and placed in another container with control diet for seven additional days. Presence of ruminant material in insect feed and in BSF larvae was assessed in five different laboratories using (i) real time-PCR analysis, (ii) multi-target ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), (iii) protein-centric immunoaffinity-LC-MS/MS, (iv) peptide-centric immunoaffinity-LC-MS/MS, (v) tandem mass spectral library matching (SLM), and (vi) compound specific amino acid analysis (CSIA). All methods investigated detected ruminant DNA or BvHb in specific insect feed media and in BSF larvae, respectively. However, each method assessed, displayed distinct shortcomings, which precluded detection of prohibited material versus non-prohibited ruminant material in some instances. Taken together, these findings indicate that detection of prohibited material in the insect-PAP feed chain requires a tiered combined use of complementary molecular analysis approaches. We therefore advocate the use of a combined multi-tier molecular analysis suite for the detection, differentiation and tracing of prohibited material in insect-PAP based feed chains and endorse ongoing efforts to extend the currently available battery of PAP detection approaches with MS based techniques and possibly δ13CAA fingerprinting.

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Language(s): eng - English
 Dates: 2021-04-28
 Publication Status: Published online
 Pages: 29
 Publishing info: -
 Table of Contents: 1. Introduction
2. Materials and methods
2.1. Feed preparation
2.2. Rearing of BSF larvae and sample preparation
2.3. Detection of bovine hemoglobin in the feeding media and in BSF larvae
2.3.1. Real time-PCR (laboratories A and B)
2.3.2. Multi-target UHPLC-MS/MS (laboratory A)
2.3.3. Protein-centric immunoaffinity LC-MS/MS (laboratory B)
2.3.4. Peptide-centric immunoaffinity LC-MS/MS (laboratory C)
2.3.5. Spectral library matching (laboratory D)
2.3.6. Stable isotope analyses (laboratory E)
3. Results and discussion
3.1. Black soldier fly larvae development
3.2. Detection of bovine hemoglobin powder in the feeding media and in BSF larvae
3.2.1. qPCR
3.2.2. LC-MS/MS-based approaches
3.2. 3 δ 13CAA fingerprinting method
4. Conclusions
 Rev. Type: No review
 Identifiers: DOI: 10.1016/j.foodcont.2021.108183
Other: shh2913
 Degree: -

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Title: Food control
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: 108183 Start / End Page: - Identifier: ISSN: 0956-7135
ISSN: 1873-7129
CoNE: https://pure.mpg.de/cone/journals/resource/0956-7135