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  Chloroplast Transformation in Arabidopsis

Ruf, S., Kroop, X., & Bock, R. (2021). Chloroplast Transformation in Arabidopsis. Current Protocols in Plant Biology, 1(4): e103. doi:10.1002/cpz1.103.

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Genre: Journal Article
Alternative Title : Current Protocols

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 Creators:
Ruf, S.1, Author              
Kroop, X.1, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: Arabidopsis, biolistic transformation, chloroplast transformation, plastid transformation, transplastomic plant
 Abstract: Abstract Plastids (chloroplasts) are the defining organelles of plants and eukaryotic algae. In addition to performing photosynthesis, plastids harbor numerous other metabolic pathways and therefore are often referred to as the biosynthetic center of the plant cell. The chloroplasts of seed plants possess dozens of copies of a circular genome of ?150 kb that contains a conserved set of 120 to 130 genes. The engineering of this genome by genetic transformation is technically challenging and currently only possible in a small number of species. In this article, we describe the methods involved in generating stable chloroplast-transformed (transplastomic) plants in the model species Arabidopsis (Arabidopsis thaliana). The protocols presented here can be applied to (1) target genes in the Arabidopsis chloroplast genome by reverse genetics and (2) express reporter genes or other foreign genes of interest in plastids of Arabidopsis plants. ? 2021 The Authors. Basic Protocol 1: Generation of root-derived microcallus material for biolistic transformation Basic Protocol 2: Chloroplast transformation by biolistic bombardment of root-derived microcalli Basic Protocol 3: Regeneration of transplastomic lines and seed production

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1002/cpz1.103
 Degree: -

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Title: Current Protocols in Plant Biology
  Abbreviation : Curr Protoc Plant Biol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Chichester : Wiley
Pages: - Volume / Issue: 1 (4) Sequence Number: e103 Start / End Page: - Identifier: ISSN: 2379-8068
CoNE: https://pure.mpg.de/cone/journals/resource/2379-8068