ausblenden:
Schlagwörter:
Deconvolution; Flickering; Fully automatic; Hidden Markov models; Homogeneous and heterogeneous noise; Ion channel recordings; Low-pass filtering; Open-channel noise; PorB; Subconductance states
Zusammenfassung:
Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other.
