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  Selected tools to visualize membrane interactions

Grothe, T., Nowak, J., Jahn, R., & Walla, P. J. (2021). Selected tools to visualize membrane interactions. European Biophysics Journal, 50(2), 211-222. doi:10.1007/s00249-021-01516-6.

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 Creators:
Grothe, T.1, Author              
Nowak, J., Author
Jahn, R.2, Author              
Walla, P. J., Author
Affiliations:
1Laboratory of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_3049887              
2Laboratory of Neurobiology, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_3049887              

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Free keywords: Fluorescence cross-correlation spectroscopy; Fluorescence lifetime analysis; Liposomes; Membrane fusion intermediates; Synaptic proteins
 Abstract: In the past decade, we developed various fluorescence-based methods for monitoring membrane fusion, membrane docking, distances between membranes, and membrane curvature. These tools were mainly developed using liposomes as model systems, which allows for the dissection of specific interactions mediated by, for example, fusion proteins. Here, we provide an overview of these methods, including two-photon fluorescence cross-correlation spectroscopy and intramembrane Förster energy transfer, with asymmetric labelling of inner and outer membrane leaflets and the calibrated use of transmembrane energy transfer to determine membrane distances below 10 nm. We discuss their application range and their limitations using examples from our work on protein-mediated vesicle docking and fusion.

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Language(s): eng - English
 Dates: 2021-03-312021-03
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s00249-021-01516-6
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Title: European Biophysics Journal
Source Genre: Journal
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Pages: - Volume / Issue: 50 (2) Sequence Number: - Start / End Page: 211 - 222 Identifier: -