ausblenden:
Schlagwörter:
-
Zusammenfassung:
Conventional analytical procedures only permit the determination of average concentrations of cell constituents in many cells. In contrast, the electron microprobe allows one to measure the amounts of the various elements present in individual cells. However, the application of the microprobe encounters the difficulty that during the preparation of the cells for analysis uncontrolled losses of diffusible cell constituents such as K and Na may take place. In the present paper an attempt was made to design a technique which minimizes such losses. A standardization procedure was applied to assess the success of the preparation procedure. This involved the preparation of cells of varying K and Na contents and a comparison of the averages of the K and Na X-ray intensities as measured in many single cells by means of the electron microprobe with the average K and Na contents of the population as determined by flame photometry.
